PDT of rat mammary adenocarcinoma in vitro and in a rat dorsal skin flap window chamber using Photofrin and chloroaluminum sulfonated phthalocyanine

Stephen T. Flock, Steven Jacques, Susan M. Small, Scott J. Stern

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photodynamic therapy has been shown to directly kill cancer cells in vitro. PDT can also kill cancer tumors in vivo, although in such a case the tumorcidal effect may be a result of several mechanisms rather than direct cancer cell kill alone. For example, PDT usually causes an inhibition in blood flow in the treatment volume and it is conceivable that such an effect might compromise the tumor. In investigating the behavior and mechanisms of PDT in vitro and in vivo, we performed PDT on a rat mammary adenocarcinoma (MTF7) both in tissue culture and in an animal model. The animal experiments were done using a rat dorsal skin flap window chamber in which a solid tumor was grown. The window chamber model allowed us to observe, over a period of weeks, the growth of the tumor and the effect of PDT on the tumor and vasculature. We utilized two different photosensitizing dyes in this study- Photofrin and chloroaluminum sulfonated phthalocyanine. In order to compare the tumorcidal efficacy of the photosensitizers in vivo and in-vitro, we quantitated the photosensitizer present in the cancer cells by measuring their fluorescence.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsSteven L. Jacques, Abraham Katzir
PublisherPubl by Int Soc for Optical Engineering
Pages77-89
Number of pages13
Volume1427
StatePublished - 1991
Externally publishedYes
EventProceedings of Laser-Tissue Interaction II - Los Angeles, CA, USA
Duration: Jan 21 1991Jan 23 1991

Other

OtherProceedings of Laser-Tissue Interaction II
CityLos Angeles, CA, USA
Period1/21/911/23/91

Fingerprint

rats
Rats
Tumors
Skin
tumors
chambers
cancer
Photosensitizers
Cells
Animals
Tissue culture
Photodynamic therapy
animal models
blood flow
animals
therapy
Blood
Dyes
dyes
Fluorescence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Flock, S. T., Jacques, S., Small, S. M., & Stern, S. J. (1991). PDT of rat mammary adenocarcinoma in vitro and in a rat dorsal skin flap window chamber using Photofrin and chloroaluminum sulfonated phthalocyanine. In S. L. Jacques, & A. Katzir (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1427, pp. 77-89). Publ by Int Soc for Optical Engineering.

PDT of rat mammary adenocarcinoma in vitro and in a rat dorsal skin flap window chamber using Photofrin and chloroaluminum sulfonated phthalocyanine. / Flock, Stephen T.; Jacques, Steven; Small, Susan M.; Stern, Scott J.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Steven L. Jacques; Abraham Katzir. Vol. 1427 Publ by Int Soc for Optical Engineering, 1991. p. 77-89.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Flock, ST, Jacques, S, Small, SM & Stern, SJ 1991, PDT of rat mammary adenocarcinoma in vitro and in a rat dorsal skin flap window chamber using Photofrin and chloroaluminum sulfonated phthalocyanine. in SL Jacques & A Katzir (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1427, Publ by Int Soc for Optical Engineering, pp. 77-89, Proceedings of Laser-Tissue Interaction II, Los Angeles, CA, USA, 1/21/91.
Flock ST, Jacques S, Small SM, Stern SJ. PDT of rat mammary adenocarcinoma in vitro and in a rat dorsal skin flap window chamber using Photofrin and chloroaluminum sulfonated phthalocyanine. In Jacques SL, Katzir A, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1427. Publ by Int Soc for Optical Engineering. 1991. p. 77-89
Flock, Stephen T. ; Jacques, Steven ; Small, Susan M. ; Stern, Scott J. / PDT of rat mammary adenocarcinoma in vitro and in a rat dorsal skin flap window chamber using Photofrin and chloroaluminum sulfonated phthalocyanine. Proceedings of SPIE - The International Society for Optical Engineering. editor / Steven L. Jacques ; Abraham Katzir. Vol. 1427 Publ by Int Soc for Optical Engineering, 1991. pp. 77-89
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