PDGFR-A is a therapeutic target in alveolar rhabdomyosarcoma

E. Taniguchi, K. Nishijo, A. T. McCleish, J. E. Michalek, M. H. Grayson, A. J. Infante, H. E. Abboud, R. D. Legallo, S. J. Qualman, B. P. Rubin, C. Keller

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Alveolar rhabdomyosarcoma is an aggressive skeletal muscle cancer of childhood. Our initial studies of rhabdomyosarcoma gene expression for patients enrolled in a national clinical trial suggested that platelet-derived growth factor receptor A (PDGFR-A) may be a mediator of disease progression and metastasis. Using our conditional mouse tumor models that authentically recapitulate the primary mutations and metastatic progression of alveolar rhabdomyosarcomas in humans, we found by immunoblotting and immunokinase assays that PDGFR-A and its downstream effectors, mitogen-activated protein kinase and Akt, were highly activated in both primary and metastatic tumors. Inhibition of PDGFR-A by RNA interference, small molecule inhibitor or neutralizing antibody had a dramatic effect on tumor cell growth both in vitro and in vivo, although resistance evolved in one-third of tumors. These results establish proof-of-principal for PDGFR-A as a therapeutic target in alveolar rhabdomyosarcoma.

Original languageEnglish (US)
Pages (from-to)6550-6560
Number of pages11
JournalOncogene
Volume27
Issue number51
DOIs
StatePublished - Oct 30 2008

Keywords

  • Alveolar rhabdomyosarcoma
  • PDGFR-A
  • Therapeutic target

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Taniguchi, E., Nishijo, K., McCleish, A. T., Michalek, J. E., Grayson, M. H., Infante, A. J., Abboud, H. E., Legallo, R. D., Qualman, S. J., Rubin, B. P., & Keller, C. (2008). PDGFR-A is a therapeutic target in alveolar rhabdomyosarcoma. Oncogene, 27(51), 6550-6560. https://doi.org/10.1038/onc.2008.255