PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix

Cynthia L. Bethea, Thomas K. Borg

    Research output: Contribution to journalArticle

    5 Scopus citations

    Abstract

    PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.

    Original languageEnglish (US)
    Pages (from-to)113-128
    Number of pages16
    JournalMolecular and Cellular Endocrinology
    Volume58
    Issue number2-3
    DOIs
    StatePublished - Aug 1988

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    Keywords

    • Dopamine
    • PC12 cells, Extracellular matrix

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Endocrinology

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