Pausing during reverse transcription increases the rate of retroviral recombination

Christian Lanciault, James J. Champoux

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Retroviruses package two copies of genomic RNA into viral particles. During the minus-sense DNA synthesis step of reverse transcription, the nascent DNA can transfer multiple times between the two copies of the genome, resulting in recombination. The mechanism for this process is similar to the process of obligate strand transfers mediated by the repeat and primer binding site sequences. The location at which the DNA 3′ terminus completely transfers to the second RNA strand defines the point of crossover. Previous work in vitro demonstrated that reverse transcriptase pausing has a significant impact on the location of the crossover, with a proportion of complete transfer events occurring very close to pause sites. The role of pausing in vivo, however, is not clearly understood. By employing a murine leukemia virus-based single-cycle infection assay, strong pausing was shown to increase the probability of recombination, as reflected in the reconstitution of green fluorescent protein expression. The infection assay results were directly correlated with the presence of strong pause sites in reverse transcriptase primer extension assays in vitro. Conversely, when pausing was diminished in vitro, without changing the sequence of the RNA template involved in recombination, there was a significant reduction in recombination in vivo. Together, these data demonstrate that reverse transcriptase pausing, as observed in vitro, directly correlates with recombination during minus-sense DNA synthesis in vivo.

Original languageEnglish (US)
Pages (from-to)2483-2494
Number of pages12
JournalJournal of virology
Volume80
Issue number5
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Fingerprint

reverse transcription
Genetic Recombination
Reverse Transcription
RNA-directed DNA polymerase
RNA-Directed DNA Polymerase
DNA
Retroviridae
assays
Murine leukemia virus
RNA
synthesis
Murine Leukemia Viruses
virion
green fluorescent protein
infection
binding sites
Green Fluorescent Proteins
Infection
protein synthesis
Virion

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Pausing during reverse transcription increases the rate of retroviral recombination. / Lanciault, Christian; Champoux, James J.

In: Journal of virology, Vol. 80, No. 5, 01.03.2006, p. 2483-2494.

Research output: Contribution to journalArticle

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