Patterns of genomic sequence diversity among their simian immunodeficiency viruses suggest that L'Hoest monkeys (Cercopithecus lhoesti) are a natural lentivirus reservoir

Brigitte E. Beer, Elizabeth Bailes, George Dapolito, Barbara J. Campbell, Robert M. Goeken, Michael Axthelm, Philip D. Markham, Jacky Bernard, Daniel Zagury, Genoveffa Franchini, Paul M. Sharp, Vanessa M. Hirsch

    Research output: Contribution to journalArticle

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    Abstract

    Recently, we described a novel simian immunodeficiency virus (SIVlhoest) from a wild-caught L'Hoest monkey (Cercopithecus lhoesti) from a North American zoo. To investigate whether L'Hoest monkeys are the natural host for these viruses, we have screened blood samples from 14 wild animals from the Democratic Republic of Congo. Eight (57%) were found to be seropositive for SIV. Nearly full-length genome sequences were obtained for SIV isolates from three of these monkeys and compared to the original isolate and to other SIVs. The four samples of SMhoest formed a distinct cluster in phylogenetic trees. Two of these isolates differed on average at only about 5% of nucleotides, suggesting that they were epidemiologically linked; otherwise, the SIVlhoest isolates differed on average by 18%. Both the level of diversity and the pattern of its variation along the genome were very similar to those seen among isolates of SIVagm from vervet monkeys, pointing to similarities in the nature of, and constraints on, SIV evolution in these two species. Discordant phylogenetic relationships among the SIVlhoest isolates for different genomic regions indicated that mosaic viruses have been generated by recombination, implying that individual monkeys have been coinfected by more than one strain of SIV. Taken together, these observations provide strong evidence that L'Hoest monkeys constitute a natural reservoir for SIV.

    Original languageEnglish (US)
    Pages (from-to)3892-3898
    Number of pages7
    JournalJournal of Virology
    Volume74
    Issue number8
    DOIs
    StatePublished - 2000

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    Cercopithecus
    Simian immunodeficiency virus
    Simian Immunodeficiency Virus
    Lentivirus
    Haplorhini
    monkeys
    genomics
    Genome
    Democratic Republic of the Congo
    Mosaic Viruses
    Cercopithecus aethiops
    viruses
    Wild Animals
    genome
    phylogeny
    wild animals
    zoos
    Genetic Recombination
    Nucleotides
    nucleotides

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Patterns of genomic sequence diversity among their simian immunodeficiency viruses suggest that L'Hoest monkeys (Cercopithecus lhoesti) are a natural lentivirus reservoir. / Beer, Brigitte E.; Bailes, Elizabeth; Dapolito, George; Campbell, Barbara J.; Goeken, Robert M.; Axthelm, Michael; Markham, Philip D.; Bernard, Jacky; Zagury, Daniel; Franchini, Genoveffa; Sharp, Paul M.; Hirsch, Vanessa M.

    In: Journal of Virology, Vol. 74, No. 8, 2000, p. 3892-3898.

    Research output: Contribution to journalArticle

    Beer, BE, Bailes, E, Dapolito, G, Campbell, BJ, Goeken, RM, Axthelm, M, Markham, PD, Bernard, J, Zagury, D, Franchini, G, Sharp, PM & Hirsch, VM 2000, 'Patterns of genomic sequence diversity among their simian immunodeficiency viruses suggest that L'Hoest monkeys (Cercopithecus lhoesti) are a natural lentivirus reservoir', Journal of Virology, vol. 74, no. 8, pp. 3892-3898. https://doi.org/10.1128/JVI.74.8.3892-3898.2000
    Beer, Brigitte E. ; Bailes, Elizabeth ; Dapolito, George ; Campbell, Barbara J. ; Goeken, Robert M. ; Axthelm, Michael ; Markham, Philip D. ; Bernard, Jacky ; Zagury, Daniel ; Franchini, Genoveffa ; Sharp, Paul M. ; Hirsch, Vanessa M. / Patterns of genomic sequence diversity among their simian immunodeficiency viruses suggest that L'Hoest monkeys (Cercopithecus lhoesti) are a natural lentivirus reservoir. In: Journal of Virology. 2000 ; Vol. 74, No. 8. pp. 3892-3898.
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