Patterns of brain activation associated with contextual conditioning to methamphetamine in mice

Justin S. Rhodes, Andrey E. Ryabinin, John C. Crabbe

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Classical conditioning is thought to play a key role in addiction. The authors used c-Fos immunohistochemistry to demonstrate a conditioned physiological response to methamphetamine (meth) in mice. Male outbred mice were placed into an environment where they had previously experienced 2 mg/kg meth or saline. The meth-paired mice displayed increased c-Fos in several brain regions, including the nucleus accumbens, prefrontal cortex, orbitofrontal cortex, basolateral amygdala, and bed nucleus of the stria terminalis. No conditioned locomotor activity was observed, but individual activity levels strongly correlated with c-Fos in many regions. A batch effect among immunohistochemical assays was demonstrated. Results implicate specific brain regions in classical conditioning to meth and demonstrate the importance of considering locomotor activity and batch in a c-Fos study.

Original languageEnglish (US)
Pages (from-to)759-771
Number of pages13
JournalBehavioral Neuroscience
Volume119
Issue number3
DOIs
StatePublished - Jun 2005

Keywords

  • Classical conditioning
  • Locomotor activity
  • Methamphetamine
  • Mice
  • c-Fos

ASJC Scopus subject areas

  • Behavioral Neuroscience

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