Patterns of brain activation associated with contextual conditioning to methamphetamine in mice

Justin S. Rhodes, Andrey Ryabinin, John Jr Crabbe

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Classical conditioning is thought to play a key role in addiction. The authors used c-Fos immunohistochemistry to demonstrate a conditioned physiological response to methamphetamine (meth) in mice. Male outbred mice were placed into an environment where they had previously experienced 2 mg/kg meth or saline. The meth-paired mice displayed increased c-Fos in several brain regions, including the nucleus accumbens, prefrontal cortex, orbitofrontal cortex, basolateral amygdala, and bed nucleus of the stria terminalis. No conditioned locomotor activity was observed, but individual activity levels strongly correlated with c-Fos in many regions. A batch effect among immunohistochemical assays was demonstrated. Results implicate specific brain regions in classical conditioning to meth and demonstrate the importance of considering locomotor activity and batch in a c-Fos study.

Original languageEnglish (US)
Pages (from-to)759-771
Number of pages13
JournalBehavioral Neuroscience
Volume119
Issue number3
DOIs
StatePublished - Jun 2005
Externally publishedYes

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Methamphetamine
Classical Conditioning
Brain
Locomotion
Prefrontal Cortex
Septal Nuclei
Nucleus Accumbens
Immunohistochemistry
Conditioning (Psychology)

Keywords

  • c-Fos
  • Classical conditioning
  • Locomotor activity
  • Methamphetamine
  • Mice

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Clinical Psychology

Cite this

Patterns of brain activation associated with contextual conditioning to methamphetamine in mice. / Rhodes, Justin S.; Ryabinin, Andrey; Crabbe, John Jr.

In: Behavioral Neuroscience, Vol. 119, No. 3, 06.2005, p. 759-771.

Research output: Contribution to journalArticle

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