Pathogenesis of Cryptococcus neoformans is associated with quantitative differences in multiple virulence factors

Rebecca Blackstock, Kent L. Buchanan, Robert Cherniak, Thomas G. Mitchell, Brian Wong, Ann Bartiss, Lydgia Jackson, Juneann W. Murphy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Two isolates of Cryptococcus neoformans were previously described as being highly divergent in their level of capsule synthesis in vivo and in their virulence for mice. The highly virulent isolate (NU-2) produced more capsule than a weakly virulent isolate (184A) in vitro under tissue culture conditions and in vivo. This investigation was done to determine if there were differences between the two isolates in other factors that might also contribute to virulence. Growth rate was not a factor as NU-2 grew more slowly than 184A. Based on PCR fingerprinting the two isolates were genetically different providing an opportunity to examine differences in multiple virulence traits. Quantitative analysis revealed that NU-2 expressed significantly more melanin and mannitol than did 184A. Although the isolates expressed the same capsular chemotype, NU-2 produced an additional structure reporter group (SRG) under tissue culture conditions that was not present when grown in glucose salts/urea/basal medium (GSU). Capsular polysaccharide SRGs of 184A were unaffected by shifting the growth conditions from GSU to tissue culture conditions. Our results suggest that pathogenesis of a C. neoformans strain is dictated by the quantitative expression of the strain's combined virulence traits. Regulators of the expression of these genes may be playing key roles in virulence.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalMycopathologia
Volume147
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Virulence Factors
Virulence
virulence
pathogenesis
tissue culture
Capsules
Urea
Salts
urea
salts
Glucose
glucose
chemotypes
Melanins
Mannitol
melanin
Regulator Genes
Growth
mannitol

Keywords

  • Capsule
  • Cryptococcus
  • Mannitol
  • Melanin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Blackstock, R., Buchanan, K. L., Cherniak, R., Mitchell, T. G., Wong, B., Bartiss, A., ... Murphy, J. W. (1999). Pathogenesis of Cryptococcus neoformans is associated with quantitative differences in multiple virulence factors. Mycopathologia, 147(1), 1-11. https://doi.org/10.1023/A:1007041401743

Pathogenesis of Cryptococcus neoformans is associated with quantitative differences in multiple virulence factors. / Blackstock, Rebecca; Buchanan, Kent L.; Cherniak, Robert; Mitchell, Thomas G.; Wong, Brian; Bartiss, Ann; Jackson, Lydgia; Murphy, Juneann W.

In: Mycopathologia, Vol. 147, No. 1, 1999, p. 1-11.

Research output: Contribution to journalArticle

Blackstock, R, Buchanan, KL, Cherniak, R, Mitchell, TG, Wong, B, Bartiss, A, Jackson, L & Murphy, JW 1999, 'Pathogenesis of Cryptococcus neoformans is associated with quantitative differences in multiple virulence factors', Mycopathologia, vol. 147, no. 1, pp. 1-11. https://doi.org/10.1023/A:1007041401743
Blackstock, Rebecca ; Buchanan, Kent L. ; Cherniak, Robert ; Mitchell, Thomas G. ; Wong, Brian ; Bartiss, Ann ; Jackson, Lydgia ; Murphy, Juneann W. / Pathogenesis of Cryptococcus neoformans is associated with quantitative differences in multiple virulence factors. In: Mycopathologia. 1999 ; Vol. 147, No. 1. pp. 1-11.
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