Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni, the cyanobacterial symbiont of Lissoclinum patella

Eric W. Schmidt, James T. Nelson, David A. Rasko, Sebastian Sudek, Jonathan A. Eisen, Margo Haygood, Jacques Ravel

Research output: Contribution to journalArticle

392 Citations (Scopus)

Abstract

Prochloron spp. are obligate cyanobacterial symbionts of many didemnid family ascidians. It has been proposed that the cyclic peptides of the patellamide class found in didemnid extracts are synthesized by Prochloron spp., but studies in which host and symbiont cells are separated and chemically analyzed to identify the biosynthetic source have yielded inconclusive results. As part of the Prochloron didemni sequencing project, we identified patellamide biosynthetic genes and confirmed their function by heterologous expression of the whole pathway in Escherichia coli. The primary sequence of patellamides A and C is encoded on a single ORF that resembles a precursor peptide. We propose that this prepatellamide is heterocyclized to form thiazole and oxazoline rings, and the peptide is cleaved to yield the two cyclic patellamides, A and C. This work represents the full sequencing and functional expression of a marine natural-product pathway from an obligate symbiont. In addition, a related cluster was identified in Trichodesmium erythraeum IMS101, an important bloom-forming cyanobacterium.

Original languageEnglish (US)
Pages (from-to)7315-7320
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number20
DOIs
StatePublished - May 17 2005
Externally publishedYes

Fingerprint

Prochloron
Patella
Thiazoles
Urochordata
Cyclic Peptides
Peptides
Cyanobacteria
Biological Products
Open Reading Frames
Escherichia coli
Genes
microcin
patellamide A
patellamide C

Keywords

  • Ascidian
  • Genome sequencing
  • Heterocycle
  • Lantibiotic
  • Tunicate

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni, the cyanobacterial symbiont of Lissoclinum patella. / Schmidt, Eric W.; Nelson, James T.; Rasko, David A.; Sudek, Sebastian; Eisen, Jonathan A.; Haygood, Margo; Ravel, Jacques.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 20, 17.05.2005, p. 7315-7320.

Research output: Contribution to journalArticle

Schmidt, Eric W. ; Nelson, James T. ; Rasko, David A. ; Sudek, Sebastian ; Eisen, Jonathan A. ; Haygood, Margo ; Ravel, Jacques. / Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni, the cyanobacterial symbiont of Lissoclinum patella. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 20. pp. 7315-7320.
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AU - Ravel, Jacques

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