Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters

Kimberly Matulef, Francis I. Valiyaveetil

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Patch-clamp electrophysiology is the standard technique used for the high-resolution functional measurements on ion channels. While studies using patch clamp are commonly carried out following ion channel expression in a heterologous system such as Xenopus oocytes or tissue culture cells, these studies can also be carried out using ion channels reconstituted into lipid vesicles. In this chapter, we describe the methodology for reconstituting ion channels into liposomes and the procedure for the generation of unilamellar blisters from these liposomes that are suitable for patch clamp. Here, we focus on the bacterial K+ channel KcsA, although the methodologies described in this chapter should be applicable for the functional analysis of other ion channels.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-191
Number of pages11
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1684
ISSN (Print)1064-3745

Keywords

  • Blisters
  • GUV recordings
  • KcsA
  • Lipid vesicles
  • Patch clamp

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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