Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters

Kimberly Matulef, Francis I. Valiyaveetil

Research output: ResearchChapter

Abstract

Patch-clamp electrophysiology is the standard technique used for the high-resolution functional measurements on ion channels. While studies using patch clamp are commonly carried out following ion channel expression in a heterologous system such as Xenopus oocytes or tissue culture cells, these studies can also be carried out using ion channels reconstituted into lipid vesicles. In this chapter, we describe the methodology for reconstituting ion channels into liposomes and the procedure for the generation of unilamellar blisters from these liposomes that are suitable for patch clamp. Here, we focus on the bacterial K+ channel KcsA, although the methodologies described in this chapter should be applicable for the functional analysis of other ion channels.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-191
Number of pages11
Volume1684
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1684
ISSN (Print)1064-3745

Fingerprint

Blister
Ion Channels
Unilamellar Liposomes
Electrophysiology
Xenopus
Liposomes
Oocytes
Cell Culture Techniques
Lipids

Keywords

  • Blisters
  • GUV recordings
  • KcsA
  • Lipid vesicles
  • Patch clamp

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Matulef, K., & Valiyaveetil, F. I. (2018). Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters. In Methods in Molecular Biology (Vol. 1684, pp. 181-191). (Methods in Molecular Biology; Vol. 1684). Humana Press Inc.. DOI: 10.1007/978-1-4939-7362-0_14

Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters. / Matulef, Kimberly; Valiyaveetil, Francis I.

Methods in Molecular Biology. Vol. 1684 Humana Press Inc., 2018. p. 181-191 (Methods in Molecular Biology; Vol. 1684).

Research output: ResearchChapter

Matulef, K & Valiyaveetil, FI 2018, Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters. in Methods in Molecular Biology. vol. 1684, Methods in Molecular Biology, vol. 1684, Humana Press Inc., pp. 181-191. DOI: 10.1007/978-1-4939-7362-0_14
Matulef K, Valiyaveetil FI. Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters. In Methods in Molecular Biology. Vol. 1684. Humana Press Inc.2018. p. 181-191. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-7362-0_14
Matulef, Kimberly ; Valiyaveetil, Francis I./ Patch-clamp recordings of the KcsA K+ channel in unilamellar blisters. Methods in Molecular Biology. Vol. 1684 Humana Press Inc., 2018. pp. 181-191 (Methods in Molecular Biology).
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