Patch-clamp, ion-sensing, and glutamate-sensing techniques to study glutamate transport in isolated retinal glial cells

Brian Billups, Marek Szatkowski, David Rossi, David Attwell

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have described how a combination of electrical, ion-sensing, and glutamate-sensing techniques has advanced our understanding of glutamate uptake into isolated salamander retinal glial cells. The next steps in understanding glutamate transport will inevitably depend strongly on molecular biological methods, as described elsewhere in this book, but will also require more detailed study of transporters in their normal environment, perhaps by using patch-clamping or imaging techniques to study cells in situ.

Original languageEnglish (US)
Pages (from-to)617-632
Number of pages16
JournalMethods in Enzymology
Volume296
DOIs
StatePublished - Jan 1 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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