Partial MHC Constructs Treat Thromboembolic Ischemic Stroke Characterized by Early Immune Expansion

Abby L. Dotson, Yingxin Chen, Wenbin Zhu, Nicole Libal, Nabil Alkayed, Halina Offner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Inflammation and thrombosis are tightly linked, with inflammation contributing to thromboembolism and to stroke outcome. Thromboembolism is a frequent cause of ischemic stroke; yet, the most used occlusion mouse models of experimental stroke do not effectively replicate thromboembolism. Our group recently described a novel thromboembolic mouse model of stroke that successfully occludes the middle cerebral artery with high reproducibility. In the current study, we characterize the peripheral and local immune outcomes as well as the ischemic response to immune therapy in a clinically relevant mouse model of thromboembolic stroke. Brain and spleen tissues were harvested 24 h after thromboembolic stroke and cells immunophenotyped by flow cytometry. We observed a significant increase in neutrophils and early activated T cells in the spleen and an increase in neutrophils and activated monocytes/microglia in the ischemic cortex after thromboembolic stroke. Moreover, as was shown previously for transient MCAO models, treatment of thromboembolic stroke with partial MHC constructs significantly reduced ischemic damage indicating an equivalent effect of this immune-based therapy in the thromboembolic model that better mimics the pathophysiology of human stroke.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalTranslational Stroke Research
DOIs
StateAccepted/In press - Dec 1 2015

Fingerprint

Stroke
Thromboembolism
Neutrophils
Spleen
Inflammation
Middle Cerebral Artery
Microglia
Monocytes
Flow Cytometry
Thrombosis
Theoretical Models
T-Lymphocytes
Brain
Therapeutics

Keywords

  • Immune response
  • Neuroinflammation
  • RTL1000
  • Therapy
  • Thromboembolic stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Cardiology and Cardiovascular Medicine

Cite this

Partial MHC Constructs Treat Thromboembolic Ischemic Stroke Characterized by Early Immune Expansion. / Dotson, Abby L.; Chen, Yingxin; Zhu, Wenbin; Libal, Nicole; Alkayed, Nabil; Offner, Halina.

In: Translational Stroke Research, 01.12.2015, p. 1-9.

Research output: Contribution to journalArticle

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