Paired motor neuron-muscle recordings in zebrafish test the receptor blockade model for shaping synaptic current

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The transparent spinal cord and electrically compact fast muscle of zebrafish offer the first opportunity to perform simultaneous whole-cell patch-clamp recordings from both motor neuron and target skeletal muscle in situ. Our paired recordings reveal the fastest reported kinetics for both spontaneous and evoked synaptic currents at any synapse and a large quantal size that facilitates the resolution of spontaneous synaptic currents. We used this preparation to test the recent proposal that open channel block of the acetylcholine receptor by acetylcholine modulates the kinetics and timing of transmission between nerve and muscle in larval zebrafish (Legendre et al., 2000). Contrary to the predictions of this model, we find similar delay and onset kinetics of synaptic current at positive and negative muscle membrane potentials, even after inhibition of acetylcholinesterase. In contrast, blockade of motor neuron K channels by 4-aminopyridine prolonged the action potential and introduced a significant delay and slowing of evoked synaptic currents, demonstrating our ability to measured altered transmitter release with this system. We conclude that the kinetics of neuromuscular synaptic currents in zebrafish is not governed by receptor block.

Original languageEnglish (US)
Pages (from-to)8104-8111
Number of pages8
JournalJournal of Neuroscience
Volume25
Issue number35
DOIs
StatePublished - Aug 31 2005
Externally publishedYes

Fingerprint

Motor Neurons
Zebrafish
Muscles
4-Aminopyridine
Cholinergic Receptors
Acetylcholinesterase
Membrane Potentials
Synapses
Action Potentials
Acetylcholine
Spinal Cord
Skeletal Muscle

Keywords

  • Channel block
  • Cholinergic
  • K channel
  • Neuromuscular
  • Patch clamp
  • Quantal analysis
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Paired motor neuron-muscle recordings in zebrafish test the receptor blockade model for shaping synaptic current. / Wen, Hua; Brehm, Paul.

In: Journal of Neuroscience, Vol. 25, No. 35, 31.08.2005, p. 8104-8111.

Research output: Contribution to journalArticle

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