TY - JOUR
T1 - p300/CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint
AU - Stauffer, Daniel
AU - Chang, Bill
AU - Huang, Jing
AU - Dunn, Andrew
AU - Thayer, Mathew
PY - 2007/3/30
Y1 - 2007/3/30
N2 - The highly related acetyltransferases, p300 and CREB-binding protein (CBP) are coactivators of signal-responsive transcriptional activation. In addition, recent evidence suggests that p300/CBP also interacts directly with complexes that mediate DNA replication and repair. In this report, we show that loss of p300/CBP in mammalian cells results in a defect in the cell cycle arrest induced by stalled DNA replication. We demonstrate that complexes containing p300/CBP and ATR can be detected in mammalian cells, and that the downstream kinase CHK1 fails to be phosphorylated in response to stalled DNA replication in cells that lack p300/CBP. These observations broaden the roles for the p300/CBP acetyltransferases to include the modulation of chromatin structure and function during DNA metabolic events as well as for transcription.
AB - The highly related acetyltransferases, p300 and CREB-binding protein (CBP) are coactivators of signal-responsive transcriptional activation. In addition, recent evidence suggests that p300/CBP also interacts directly with complexes that mediate DNA replication and repair. In this report, we show that loss of p300/CBP in mammalian cells results in a defect in the cell cycle arrest induced by stalled DNA replication. We demonstrate that complexes containing p300/CBP and ATR can be detected in mammalian cells, and that the downstream kinase CHK1 fails to be phosphorylated in response to stalled DNA replication in cells that lack p300/CBP. These observations broaden the roles for the p300/CBP acetyltransferases to include the modulation of chromatin structure and function during DNA metabolic events as well as for transcription.
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U2 - 10.1074/jbc.M609261200
DO - 10.1074/jbc.M609261200
M3 - Article
C2 - 17272271
AN - SCOPUS:34248150576
SN - 0021-9258
VL - 282
SP - 9678
EP - 9687
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 13
ER -