p300 regulates p63 transcriptional activity

Mary MacPartlin, Shelya Zeng, Hunjoo Lee, Daniel Stauffer, Yetao Jin, Mathew (Matt) Thayer, Hua Lu

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The transcriptional co-activator p300 has been reported to regulate the tumor suppressor p53 and its ortholog p73. Here we describe a study showing that this coactivator also regulates the transcriptional function of p63. p300 bound to the N-terminal domain of p63γ, and p63γ bound to the N terminus of p300 in vitro and in cells. p300, but not its acetylase-defective mutant AT2, stimulated p63γ-dependent transcription and induction of p21 in cells, consequently leading to G1 arrest. Inversely, the ΔN-p63γ isoform as well as p300AT2 inhibited the induction of p21 by p63γ. These results suggest that p300 regulates p63-dependent transcription of p21.

Original languageEnglish (US)
Pages (from-to)30604-30610
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number34
DOIs
StatePublished - Aug 26 2005

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Acetylesterase
Tumors
Protein Isoforms
Neoplasms
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

MacPartlin, M., Zeng, S., Lee, H., Stauffer, D., Jin, Y., Thayer, M. M., & Lu, H. (2005). p300 regulates p63 transcriptional activity. Journal of Biological Chemistry, 280(34), 30604-30610. https://doi.org/10.1074/jbc.M503352200

p300 regulates p63 transcriptional activity. / MacPartlin, Mary; Zeng, Shelya; Lee, Hunjoo; Stauffer, Daniel; Jin, Yetao; Thayer, Mathew (Matt); Lu, Hua.

In: Journal of Biological Chemistry, Vol. 280, No. 34, 26.08.2005, p. 30604-30610.

Research output: Contribution to journalArticle

MacPartlin, M, Zeng, S, Lee, H, Stauffer, D, Jin, Y, Thayer, MM & Lu, H 2005, 'p300 regulates p63 transcriptional activity', Journal of Biological Chemistry, vol. 280, no. 34, pp. 30604-30610. https://doi.org/10.1074/jbc.M503352200
MacPartlin M, Zeng S, Lee H, Stauffer D, Jin Y, Thayer MM et al. p300 regulates p63 transcriptional activity. Journal of Biological Chemistry. 2005 Aug 26;280(34):30604-30610. https://doi.org/10.1074/jbc.M503352200
MacPartlin, Mary ; Zeng, Shelya ; Lee, Hunjoo ; Stauffer, Daniel ; Jin, Yetao ; Thayer, Mathew (Matt) ; Lu, Hua. / p300 regulates p63 transcriptional activity. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 34. pp. 30604-30610.
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