p300 regulates p63 transcriptional activity

Mary MacPartlin, Shelya Zeng, Hunjoo Lee, Daniel Stauffer, Yetao Jin, Mathew Thayer, Hua Lu

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The transcriptional co-activator p300 has been reported to regulate the tumor suppressor p53 and its ortholog p73. Here we describe a study showing that this coactivator also regulates the transcriptional function of p63. p300 bound to the N-terminal domain of p63γ, and p63γ bound to the N terminus of p300 in vitro and in cells. p300, but not its acetylase-defective mutant AT2, stimulated p63γ-dependent transcription and induction of p21 in cells, consequently leading to G1 arrest. Inversely, the ΔN-p63γ isoform as well as p300AT2 inhibited the induction of p21 by p63γ. These results suggest that p300 regulates p63-dependent transcription of p21.

Original languageEnglish (US)
Pages (from-to)30604-30610
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number34
DOIs
StatePublished - Aug 26 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    MacPartlin, M., Zeng, S., Lee, H., Stauffer, D., Jin, Y., Thayer, M., & Lu, H. (2005). p300 regulates p63 transcriptional activity. Journal of Biological Chemistry, 280(34), 30604-30610. https://doi.org/10.1074/jbc.M503352200