P2X2 receptors on ganglion and amacrine cells in cone pathways of the rat retina

Teresa Puthussery, Erica L. Fletcher

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Extracellular ATP is known to mediate fast, excitatory neurotransmission through activation of ionotropic P2X receptors. In this study, the localization of the P2X2 receptor (P2X2R) subunit was studied in rat retina by using immunofluorescence immunohistochemistry and preembedding immunoelectron microscopy. The P2X2R was observed in large ganglion cells as well as in a subset of amacrine cells. Double labeling revealed that 96% of all P2X2R-immunoreactive amacrine cells showed γ-aminobutyric acid (GABA) immunoreactivity. Subsets of P2X 2R-immunoreactive amacrine cells expressed nitric oxide synthase and substance P; however, no colocalization was observed with choline acetyltransferase, vasoactive intestinal peptide, or tyrosine hydroxylase. Nearest-neighbor analysis confirmed that P2X2Rs were expressed by a heterogeneous population of amacrine cells. The synaptic connectivity of P2X2R amacrine cells was also investigated. It was interesting that P2X2R-immunoreactive amacrine cell dendrites stratified in the sublaminae of the inner plexiform layer occupied by cone, but not rod bipolar cell axon terminals. Immunoelectron microscopy revealed that P2X 2-immunoreactive amacrine cell processes were associated with cone bipolar cell axon terminals as well as other conventional synapses in the inner plexiform layer. Taken together, these data provide further evidence for the involvement of extracellular ATP in neuronal signaling in the retina, particularly within cone pathways.

Original languageEnglish (US)
Pages (from-to)595-609
Number of pages15
JournalJournal of Comparative Neurology
Volume496
Issue number5
DOIs
StatePublished - Jun 10 2006
Externally publishedYes

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Purinergic P2X2 Receptors
Amacrine Cells
Ganglia
Retina
Immunoelectron Microscopy
Presynaptic Terminals
Adenosine Triphosphate
Aminobutyrates
Vertebrate Photoreceptor Cells
Choline O-Acetyltransferase
Vasoactive Intestinal Peptide
Tyrosine 3-Monooxygenase
Substance P
Dendrites
Nitric Oxide Synthase
Synaptic Transmission
Synapses
gamma-Aminobutyric Acid
Fluorescent Antibody Technique
Immunohistochemistry

Keywords

  • Bipolar cell
  • Extracellular ATP
  • GABA
  • Neurotransmitter
  • Purinergic
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

P2X2 receptors on ganglion and amacrine cells in cone pathways of the rat retina. / Puthussery, Teresa; Fletcher, Erica L.

In: Journal of Comparative Neurology, Vol. 496, No. 5, 10.06.2006, p. 595-609.

Research output: Contribution to journalArticle

Puthussery, Teresa ; Fletcher, Erica L. / P2X2 receptors on ganglion and amacrine cells in cone pathways of the rat retina. In: Journal of Comparative Neurology. 2006 ; Vol. 496, No. 5. pp. 595-609.
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