Ozone-induced airway hyperresponsiveness and loss of neuronal M2 muscarinic receptor function

A. H. Schultheis, D. J P Bassett, Allison Fryer

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The effect of acute ozone exposure on the function of efferent parasympathetic nerves, M3 muscarinic receptors on airway smooth muscle, and inhibitory M2 muscarinic receptors on the parasympathetic nerves was studied. Immediately after exposure to 2.0 ppm ozone for 4 h, guinea pigs became hyperresponsive to electrical stimulation of the vagus nerves. The normal airway response to intravenous cholinergic agonists at this time demonstrates normal M3 receptor function. M2 muscarinic receptors on the nerves, which normally inhibit release of acetylcholine, were dysfunctional after ozone exposure, as demonstrated by the failure of the muscarinic agonist pilocarpine to inhibit, and the failure of the M2 antagonist gallamine to potentiate, vagally mediated bronchoconstriction. Thus, loss of inhibitory M2 muscarinic receptor function after ozone exposure potentiates release of acetylcholine from the vagus nerves, increasing vagally mediated bronchoconstriction. By 14 days, postozone responses to vagal nerve stimulation were not different from those of air-exposed animals and the function of the neuronal M2 muscarinic receptor was normal, confirming that ozone-induced hyperresponsiveness is reversible.

Original languageEnglish (US)
Pages (from-to)1088-1097
Number of pages10
JournalJournal of Applied Physiology
Volume76
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Muscarinic M2 Receptors
Ozone
Vagus Nerve
Bronchoconstriction
Acetylcholine
Muscarinic M3 Receptors
Gallamine Triethiodide
Vagus Nerve Stimulation
Cholinergic Agonists
Muscarinic Agonists
Pilocarpine
Electric Stimulation
Smooth Muscle
Guinea Pigs
Air

Keywords

  • autoreceptors
  • ozone exposure
  • parasympathetic nerves
  • sympathetic nerves

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Ozone-induced airway hyperresponsiveness and loss of neuronal M2 muscarinic receptor function. / Schultheis, A. H.; Bassett, D. J P; Fryer, Allison.

In: Journal of Applied Physiology, Vol. 76, No. 3, 1994, p. 1088-1097.

Research output: Contribution to journalArticle

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