Oxytocin receptors in the primate ovary

Molecular identity and link to apoptosis in human granulosa cells

S. Saller, L. Kunz, Gregory Dissen, Richard Stouffer, Sergio Ojeda, D. Berg, U. Berg, A. Mayerhofer

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Background Oxytocin (OT) is produced by granulosa cells (GCs) of pre-ovulatory ovarian follicles and the corpus luteum (CL) in some mammalian species. Actions of OT in the ovary have been linked to luteinization, steroidogenesis and luteolysis. Human IVF-derived (h)GCs possess a functional OT receptor (OTR), linked to elevation of intracellular Ca2+, but molecular identity of the receptor for OT in human granulosa cells (hGCs) and down-stream consequences are not known.Method S AND Result SRT-PCR, sequencing and immunocytochemistry identified the genuine OTR in hGCs. OT (10 nM-10 μM) induced elevations of intracellular Ca2+ levels (Fluo-4 measurements), which were blocked by tocinoic acid (TA; 50 μM, a selective OTR-antagonist). Down-stream effects of OTR-activation include a concentration dependent decrease in cell viability/metabolism, manifested by reduced ATP-levels, increased caspase3/7-activity (P <0.05) and electron microscopical signs of cellular regression. TA blocked all of these changes. Immunoreactive OTR was found in the CL and GCs of large and, surprisingly, also small pre-antral follicles of the human ovary. Immunoreactive OTR in the rhesus monkey ovary was detected in primordial and growing primary follicles in the infantile ovary and in follicles at all stages of development in the adult ovary, as well as the CL: these Result s were corroborated by RT-PCR analysis of GCs excised by laser capture microdissection.Conclusion SOur study identifies genuine OTRs in human and rhesus monkey GCs. Activation by high levels of OT leads to cellular regression in hGCs. As GCs of small follicles also express OTRs, OT may have as yet unkown functions in follicular development.

    Original languageEnglish (US)
    Pages (from-to)969-976
    Number of pages8
    JournalHuman Reproduction
    Volume25
    Issue number4
    DOIs
    StatePublished - Apr 2010

    Fingerprint

    Oxytocin Receptors
    Granulosa Cells
    Primates
    Ovary
    Apoptosis
    Oxytocin
    Corpus Luteum
    Macaca mulatta
    Luteinization
    Laser Capture Microdissection
    Luteolysis
    Polymerase Chain Reaction
    Ovarian Follicle
    Cell Survival
    Adenosine Triphosphate
    Immunohistochemistry
    Electrons

    Keywords

    • Apoptosis
    • Calcium
    • Follicle
    • Oxytocin
    • Vasopressin

    ASJC Scopus subject areas

    • Rehabilitation
    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

    Oxytocin receptors in the primate ovary : Molecular identity and link to apoptosis in human granulosa cells. / Saller, S.; Kunz, L.; Dissen, Gregory; Stouffer, Richard; Ojeda, Sergio; Berg, D.; Berg, U.; Mayerhofer, A.

    In: Human Reproduction, Vol. 25, No. 4, 04.2010, p. 969-976.

    Research output: Contribution to journalArticle

    Saller, S. ; Kunz, L. ; Dissen, Gregory ; Stouffer, Richard ; Ojeda, Sergio ; Berg, D. ; Berg, U. ; Mayerhofer, A. / Oxytocin receptors in the primate ovary : Molecular identity and link to apoptosis in human granulosa cells. In: Human Reproduction. 2010 ; Vol. 25, No. 4. pp. 969-976.
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    AU - Stouffer, Richard

    AU - Ojeda, Sergio

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