Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes

Charles E. Ogburn, Mitchell S. Turker, Terrance J. Kavanagh, Christine M. Disteche, Annette C. Smith, Ken ichiro Fukuchi, George M. Martin

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Abstract

Stable, oxygen-resistant cell lines (O2R) were isolated from P19 and P19H22 (APRT hemizygote) mouse embryonic carcinoma cells by serial exposures of increasing durations to 95% O2. Neurally differentiated progeny were also oxygen-resistant. P19O2R exhibited reduced oxygenmediated micronucleation and a 10-to 20-fold reduction of the forward mutation rate at the HPRT locus in 20% O2. P19H22O2R cells showed reduced frequencies of colonies resistant to 2,6-diaminopurine. The modal karyotype of P19O2R was identical to that of a nonmodal karyotype present in the parental line [39, X,-Y, add(14)]. There was no evidence of enhanced resistance to ionizing radiation. We conclude that this general approach, when applied to pluripotent embryonic stem cells, has the potential to lead to the synthesis of antimutator strains of mice.

Original languageEnglish (US)
Pages (from-to)361-370
Number of pages10
JournalSomatic Cell and Molecular Genetics
Volume20
Issue number5
DOIs
StatePublished - Sep 1 1994

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ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Ogburn, C. E., Turker, M. S., Kavanagh, T. J., Disteche, C. M., Smith, A. C., Fukuchi, K. I., & Martin, G. M. (1994). Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes. Somatic Cell and Molecular Genetics, 20(5), 361-370. https://doi.org/10.1007/BF02257453