Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes

Charles E. Ogburn, Mitchell Turker, Terrance J. Kavanagh, Christine M. Disteche, Annette C. Smith, Ken ichiro Fukuchi, George M. Martin

Research output: Contribution to journalArticle

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Abstract

Stable, oxygen-resistant cell lines (O2R) were isolated from P19 and P19H22 (APRT hemizygote) mouse embryonic carcinoma cells by serial exposures of increasing durations to 95% O2. Neurally differentiated progeny were also oxygen-resistant. P19O2R exhibited reduced oxygenmediated micronucleation and a 10-to 20-fold reduction of the forward mutation rate at the HPRT locus in 20% O2. P19H22O2R cells showed reduced frequencies of colonies resistant to 2,6-diaminopurine. The modal karyotype of P19O2R was identical to that of a nonmodal karyotype present in the parental line [39, X,-Y, add(14)]. There was no evidence of enhanced resistance to ionizing radiation. We conclude that this general approach, when applied to pluripotent embryonic stem cells, has the potential to lead to the synthesis of antimutator strains of mice.

Original languageEnglish (US)
Pages (from-to)361-370
Number of pages10
JournalSomatic Cell and Molecular Genetics
Volume20
Issue number5
DOIs
StatePublished - Sep 1994
Externally publishedYes

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Karyotype
Hemizygote
Oxygen
Carcinoma
Phenotype
Hypoxanthine Phosphoribosyltransferase
Cell Line
Pluripotent Stem Cells
Mutation Rate
Embryonic Stem Cells
Ionizing Radiation
2,6-diaminopurine

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Ogburn, C. E., Turker, M., Kavanagh, T. J., Disteche, C. M., Smith, A. C., Fukuchi, K. I., & Martin, G. M. (1994). Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes. Somatic Cell and Molecular Genetics, 20(5), 361-370. https://doi.org/10.1007/BF02257453

Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes. / Ogburn, Charles E.; Turker, Mitchell; Kavanagh, Terrance J.; Disteche, Christine M.; Smith, Annette C.; Fukuchi, Ken ichiro; Martin, George M.

In: Somatic Cell and Molecular Genetics, Vol. 20, No. 5, 09.1994, p. 361-370.

Research output: Contribution to journalArticle

Ogburn, CE, Turker, M, Kavanagh, TJ, Disteche, CM, Smith, AC, Fukuchi, KI & Martin, GM 1994, 'Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes', Somatic Cell and Molecular Genetics, vol. 20, no. 5, pp. 361-370. https://doi.org/10.1007/BF02257453
Ogburn, Charles E. ; Turker, Mitchell ; Kavanagh, Terrance J. ; Disteche, Christine M. ; Smith, Annette C. ; Fukuchi, Ken ichiro ; Martin, George M. / Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes. In: Somatic Cell and Molecular Genetics. 1994 ; Vol. 20, No. 5. pp. 361-370.
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