TY - JOUR
T1 - Oxidative stress in systemic lupus erythematosus
T2 - Relationship to Th1 cytokine and disease activity
AU - Shah, Dilip
AU - Kiran, Ravi
AU - Wanchu, Ajay
AU - Bhatnagar, Archana
PY - 2010/3/10
Y1 - 2010/3/10
N2 - Imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines is one possible cause for the pathogenesis of systemic lupus erythematosus (SLE). To evaluate the correlation between oxidative stress and Th1 cytokine level with the disease activity of SLE in the North Indian population, oxidative/anti-oxidant profiles: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and interferon (IFN)-γ, interleukin (IL)-12 were studied. The estimation of oxidant and anti-oxidant enzymes was done in erythrocyte hemolysate and the levels of IFN-γ and IL-12 were determined in the culture supernatant of peripheral blood mononuclear cells (PBMC) by a sandwich enzyme-linked immunosorbent assay (ELISA). A significant increase in the level of lipid peroxidation, measured as malondialdehyde (MDA), was found in SLE patients. The activities of anti-oxidant enzymes: SOD, CAT, GPx and anti-oxidant molecule GSH were significantly reduced in SLE patients as compared to controls. Increased levels of IFN-γ and IL-12 were found in the culture supernatant of PBMC of SLE patients. MDA level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score, IFN-γ, IL-12 and negatively correlated with GSH in SLE patients. Strong positive correlations of IFN-γ and MDA with SLEDAI score suggest that lipid peroxidation and pro-inflammatory cytokine, both are involved in the pathogenesis of SLE. Collectively, this study advocates that severity of disease might be enhanced by imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines in SLE.
AB - Imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines is one possible cause for the pathogenesis of systemic lupus erythematosus (SLE). To evaluate the correlation between oxidative stress and Th1 cytokine level with the disease activity of SLE in the North Indian population, oxidative/anti-oxidant profiles: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and interferon (IFN)-γ, interleukin (IL)-12 were studied. The estimation of oxidant and anti-oxidant enzymes was done in erythrocyte hemolysate and the levels of IFN-γ and IL-12 were determined in the culture supernatant of peripheral blood mononuclear cells (PBMC) by a sandwich enzyme-linked immunosorbent assay (ELISA). A significant increase in the level of lipid peroxidation, measured as malondialdehyde (MDA), was found in SLE patients. The activities of anti-oxidant enzymes: SOD, CAT, GPx and anti-oxidant molecule GSH were significantly reduced in SLE patients as compared to controls. Increased levels of IFN-γ and IL-12 were found in the culture supernatant of PBMC of SLE patients. MDA level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score, IFN-γ, IL-12 and negatively correlated with GSH in SLE patients. Strong positive correlations of IFN-γ and MDA with SLEDAI score suggest that lipid peroxidation and pro-inflammatory cytokine, both are involved in the pathogenesis of SLE. Collectively, this study advocates that severity of disease might be enhanced by imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines in SLE.
KW - Erythematosus (SLE)
KW - Interferon (IFN)-γ
KW - Interleukin (IL)-12
KW - Oxidative stress
KW - Systemic lupus
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U2 - 10.1016/j.imlet.2010.01.005
DO - 10.1016/j.imlet.2010.01.005
M3 - Article
C2 - 20105444
AN - SCOPUS:77649184355
SN - 0165-2478
VL - 129
SP - 7
EP - 12
JO - Immunology Letters
JF - Immunology Letters
IS - 1
ER -