Oxidative stress in systemic lupus erythematosus

Relationship to Th1 cytokine and disease activity

Dilip Shah, Ravi Kiran, Ajay Wanchu, Archana Bhatnagar

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines is one possible cause for the pathogenesis of systemic lupus erythematosus (SLE). To evaluate the correlation between oxidative stress and Th1 cytokine level with the disease activity of SLE in the North Indian population, oxidative/anti-oxidant profiles: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and interferon (IFN)-γ, interleukin (IL)-12 were studied. The estimation of oxidant and anti-oxidant enzymes was done in erythrocyte hemolysate and the levels of IFN-γ and IL-12 were determined in the culture supernatant of peripheral blood mononuclear cells (PBMC) by a sandwich enzyme-linked immunosorbent assay (ELISA). A significant increase in the level of lipid peroxidation, measured as malondialdehyde (MDA), was found in SLE patients. The activities of anti-oxidant enzymes: SOD, CAT, GPx and anti-oxidant molecule GSH were significantly reduced in SLE patients as compared to controls. Increased levels of IFN-γ and IL-12 were found in the culture supernatant of PBMC of SLE patients. MDA level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score, IFN-γ, IL-12 and negatively correlated with GSH in SLE patients. Strong positive correlations of IFN-γ and MDA with SLEDAI score suggest that lipid peroxidation and pro-inflammatory cytokine, both are involved in the pathogenesis of SLE. Collectively, this study advocates that severity of disease might be enhanced by imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines in SLE.

Original languageEnglish (US)
Pages (from-to)7-12
Number of pages6
JournalImmunology Letters
Volume129
Issue number1
DOIs
StatePublished - Mar 10 2010
Externally publishedYes

Fingerprint

Systemic Lupus Erythematosus
Oxidative Stress
Cytokines
Oxidants
Interferons
Interleukin-12
Malondialdehyde
Glutathione Peroxidase
Helper-Inducer T-Lymphocytes
Catalase
Lipid Peroxidation
Superoxide Dismutase
Blood Cells
Enzymes
Glutathione
Erythrocytes
Enzyme-Linked Immunosorbent Assay

Keywords

  • Erythematosus (SLE)
  • Interferon (IFN)-γ
  • Interleukin (IL)-12
  • Oxidative stress
  • Systemic lupus

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Oxidative stress in systemic lupus erythematosus : Relationship to Th1 cytokine and disease activity. / Shah, Dilip; Kiran, Ravi; Wanchu, Ajay; Bhatnagar, Archana.

In: Immunology Letters, Vol. 129, No. 1, 10.03.2010, p. 7-12.

Research output: Contribution to journalArticle

Shah, Dilip ; Kiran, Ravi ; Wanchu, Ajay ; Bhatnagar, Archana. / Oxidative stress in systemic lupus erythematosus : Relationship to Th1 cytokine and disease activity. In: Immunology Letters. 2010 ; Vol. 129, No. 1. pp. 7-12.
@article{e968057b73e04a23b679b92cd4083999,
title = "Oxidative stress in systemic lupus erythematosus: Relationship to Th1 cytokine and disease activity",
abstract = "Imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines is one possible cause for the pathogenesis of systemic lupus erythematosus (SLE). To evaluate the correlation between oxidative stress and Th1 cytokine level with the disease activity of SLE in the North Indian population, oxidative/anti-oxidant profiles: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and interferon (IFN)-γ, interleukin (IL)-12 were studied. The estimation of oxidant and anti-oxidant enzymes was done in erythrocyte hemolysate and the levels of IFN-γ and IL-12 were determined in the culture supernatant of peripheral blood mononuclear cells (PBMC) by a sandwich enzyme-linked immunosorbent assay (ELISA). A significant increase in the level of lipid peroxidation, measured as malondialdehyde (MDA), was found in SLE patients. The activities of anti-oxidant enzymes: SOD, CAT, GPx and anti-oxidant molecule GSH were significantly reduced in SLE patients as compared to controls. Increased levels of IFN-γ and IL-12 were found in the culture supernatant of PBMC of SLE patients. MDA level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score, IFN-γ, IL-12 and negatively correlated with GSH in SLE patients. Strong positive correlations of IFN-γ and MDA with SLEDAI score suggest that lipid peroxidation and pro-inflammatory cytokine, both are involved in the pathogenesis of SLE. Collectively, this study advocates that severity of disease might be enhanced by imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines in SLE.",
keywords = "Erythematosus (SLE), Interferon (IFN)-γ, Interleukin (IL)-12, Oxidative stress, Systemic lupus",
author = "Dilip Shah and Ravi Kiran and Ajay Wanchu and Archana Bhatnagar",
year = "2010",
month = "3",
day = "10",
doi = "10.1016/j.imlet.2010.01.005",
language = "English (US)",
volume = "129",
pages = "7--12",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Oxidative stress in systemic lupus erythematosus

T2 - Relationship to Th1 cytokine and disease activity

AU - Shah, Dilip

AU - Kiran, Ravi

AU - Wanchu, Ajay

AU - Bhatnagar, Archana

PY - 2010/3/10

Y1 - 2010/3/10

N2 - Imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines is one possible cause for the pathogenesis of systemic lupus erythematosus (SLE). To evaluate the correlation between oxidative stress and Th1 cytokine level with the disease activity of SLE in the North Indian population, oxidative/anti-oxidant profiles: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and interferon (IFN)-γ, interleukin (IL)-12 were studied. The estimation of oxidant and anti-oxidant enzymes was done in erythrocyte hemolysate and the levels of IFN-γ and IL-12 were determined in the culture supernatant of peripheral blood mononuclear cells (PBMC) by a sandwich enzyme-linked immunosorbent assay (ELISA). A significant increase in the level of lipid peroxidation, measured as malondialdehyde (MDA), was found in SLE patients. The activities of anti-oxidant enzymes: SOD, CAT, GPx and anti-oxidant molecule GSH were significantly reduced in SLE patients as compared to controls. Increased levels of IFN-γ and IL-12 were found in the culture supernatant of PBMC of SLE patients. MDA level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score, IFN-γ, IL-12 and negatively correlated with GSH in SLE patients. Strong positive correlations of IFN-γ and MDA with SLEDAI score suggest that lipid peroxidation and pro-inflammatory cytokine, both are involved in the pathogenesis of SLE. Collectively, this study advocates that severity of disease might be enhanced by imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines in SLE.

AB - Imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines is one possible cause for the pathogenesis of systemic lupus erythematosus (SLE). To evaluate the correlation between oxidative stress and Th1 cytokine level with the disease activity of SLE in the North Indian population, oxidative/anti-oxidant profiles: malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and interferon (IFN)-γ, interleukin (IL)-12 were studied. The estimation of oxidant and anti-oxidant enzymes was done in erythrocyte hemolysate and the levels of IFN-γ and IL-12 were determined in the culture supernatant of peripheral blood mononuclear cells (PBMC) by a sandwich enzyme-linked immunosorbent assay (ELISA). A significant increase in the level of lipid peroxidation, measured as malondialdehyde (MDA), was found in SLE patients. The activities of anti-oxidant enzymes: SOD, CAT, GPx and anti-oxidant molecule GSH were significantly reduced in SLE patients as compared to controls. Increased levels of IFN-γ and IL-12 were found in the culture supernatant of PBMC of SLE patients. MDA level was positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score, IFN-γ, IL-12 and negatively correlated with GSH in SLE patients. Strong positive correlations of IFN-γ and MDA with SLEDAI score suggest that lipid peroxidation and pro-inflammatory cytokine, both are involved in the pathogenesis of SLE. Collectively, this study advocates that severity of disease might be enhanced by imbalance between oxidative stress and helper T-cell (Th1)-derived cytokines in SLE.

KW - Erythematosus (SLE)

KW - Interferon (IFN)-γ

KW - Interleukin (IL)-12

KW - Oxidative stress

KW - Systemic lupus

UR - http://www.scopus.com/inward/record.url?scp=77649184355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649184355&partnerID=8YFLogxK

U2 - 10.1016/j.imlet.2010.01.005

DO - 10.1016/j.imlet.2010.01.005

M3 - Article

VL - 129

SP - 7

EP - 12

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 1

ER -