OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas

Mary Clare Sarff; David Edwards; Birat Dhungel; Keith W. Wegmann; Christopher Corless; Andrew D. Weinberg; John T. Vetto

doi:10.1016/j.amjsurg.2007.12.036

OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas

Mary Clare Sarff, David Edwards, Birat Dhungel, Keith W. Wegmann, Christopher Corless, Andrew D. Weinberg, John T. Vetto

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Background: The expression of OX40 (CD134) on activated CD4+ T cells has been associated with favorable cancer patient outcomes. Because of recent reports that sentinel lymph nodes (SLNs) may represent an immunosuppressive environment, we investigated the expression of OX40 in SLNs from patients with primary cutaneous melanoma. Methods: Samples of peripheral blood lymphocytes and a section of 71 SLNs from 53 patients with clinically node negative melanoma were purified for CD4+ T cells, stained for OX40, and analyzed by flow cytometry. Results: The mean percentage of OX40 on CD4 T cells in the SLNs versus peripheral blood lymphocytes was related indirectly to the T stage of the primary tumor and was decreased in ulcerated primary tumors and positive sentinel nodes. Conclusions: The expression of OX40 on CD4+ T cells in SLNs draining primary melanomas decreased with more advanced tumor features (higher T stage, ulceration) and nodal involvement, suggesting that such tumors may have an immunosuppressive effect on the SLN microenvironment.

Original language	English (US)
Pages (from-to)	621-625
Number of pages	5
Journal	American journal of surgery
Volume	195
Issue number	5
DOIs	https://doi.org/10.1016/j.amjsurg.2007.12.036
State	Published - May 2008

Keywords

Immunosuppressive environment
Melanoma
OX40
Sentinel lymph nodes

ASJC Scopus subject areas

Surgery

Access to Document

10.1016/j.amjsurg.2007.12.036

Cite this

@article{452300067df24c579d34597ca093df80,

title = "OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas",

abstract = "Background: The expression of OX40 (CD134) on activated CD4+ T cells has been associated with favorable cancer patient outcomes. Because of recent reports that sentinel lymph nodes (SLNs) may represent an immunosuppressive environment, we investigated the expression of OX40 in SLNs from patients with primary cutaneous melanoma. Methods: Samples of peripheral blood lymphocytes and a section of 71 SLNs from 53 patients with clinically node negative melanoma were purified for CD4+ T cells, stained for OX40, and analyzed by flow cytometry. Results: The mean percentage of OX40 on CD4 T cells in the SLNs versus peripheral blood lymphocytes was related indirectly to the T stage of the primary tumor and was decreased in ulcerated primary tumors and positive sentinel nodes. Conclusions: The expression of OX40 on CD4+ T cells in SLNs draining primary melanomas decreased with more advanced tumor features (higher T stage, ulceration) and nodal involvement, suggesting that such tumors may have an immunosuppressive effect on the SLN microenvironment.",

keywords = "Immunosuppressive environment, Melanoma, OX40, Sentinel lymph nodes",

author = "Sarff, {Mary Clare} and David Edwards and Birat Dhungel and Wegmann, {Keith W.} and Christopher Corless and Weinberg, {Andrew D.} and Vetto, {John T.}",

year = "2008",

month = may,

doi = "10.1016/j.amjsurg.2007.12.036",

language = "English (US)",

volume = "195",

pages = "621--625",

journal = "American journal of surgery",

issn = "0002-9610",

publisher = "Elsevier Inc.",

number = "5",

}

TY - JOUR

T1 - OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas

AU - Sarff, Mary Clare

AU - Edwards, David

AU - Dhungel, Birat

AU - Wegmann, Keith W.

AU - Corless, Christopher

AU - Weinberg, Andrew D.

AU - Vetto, John T.

PY - 2008/5

Y1 - 2008/5

N2 - Background: The expression of OX40 (CD134) on activated CD4+ T cells has been associated with favorable cancer patient outcomes. Because of recent reports that sentinel lymph nodes (SLNs) may represent an immunosuppressive environment, we investigated the expression of OX40 in SLNs from patients with primary cutaneous melanoma. Methods: Samples of peripheral blood lymphocytes and a section of 71 SLNs from 53 patients with clinically node negative melanoma were purified for CD4+ T cells, stained for OX40, and analyzed by flow cytometry. Results: The mean percentage of OX40 on CD4 T cells in the SLNs versus peripheral blood lymphocytes was related indirectly to the T stage of the primary tumor and was decreased in ulcerated primary tumors and positive sentinel nodes. Conclusions: The expression of OX40 on CD4+ T cells in SLNs draining primary melanomas decreased with more advanced tumor features (higher T stage, ulceration) and nodal involvement, suggesting that such tumors may have an immunosuppressive effect on the SLN microenvironment.

AB - Background: The expression of OX40 (CD134) on activated CD4+ T cells has been associated with favorable cancer patient outcomes. Because of recent reports that sentinel lymph nodes (SLNs) may represent an immunosuppressive environment, we investigated the expression of OX40 in SLNs from patients with primary cutaneous melanoma. Methods: Samples of peripheral blood lymphocytes and a section of 71 SLNs from 53 patients with clinically node negative melanoma were purified for CD4+ T cells, stained for OX40, and analyzed by flow cytometry. Results: The mean percentage of OX40 on CD4 T cells in the SLNs versus peripheral blood lymphocytes was related indirectly to the T stage of the primary tumor and was decreased in ulcerated primary tumors and positive sentinel nodes. Conclusions: The expression of OX40 on CD4+ T cells in SLNs draining primary melanomas decreased with more advanced tumor features (higher T stage, ulceration) and nodal involvement, suggesting that such tumors may have an immunosuppressive effect on the SLN microenvironment.

KW - Immunosuppressive environment

KW - Melanoma

KW - OX40

KW - Sentinel lymph nodes

UR - http://www.scopus.com/inward/record.url?scp=42049122108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42049122108&partnerID=8YFLogxK

U2 - 10.1016/j.amjsurg.2007.12.036

DO - 10.1016/j.amjsurg.2007.12.036

M3 - Article

C2 - 18374895

AN - SCOPUS:42049122108

SN - 0002-9610

VL - 195

SP - 621

EP - 625

JO - American journal of surgery

JF - American journal of surgery

IS - 5

ER -

OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this