OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas

Mary Clare Sarff, David Edwards, Birat Dhungel, Keith W. Wegmann, Christopher Corless, Andrew D. Weinberg, John T. Vetto

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Background: The expression of OX40 (CD134) on activated CD4+ T cells has been associated with favorable cancer patient outcomes. Because of recent reports that sentinel lymph nodes (SLNs) may represent an immunosuppressive environment, we investigated the expression of OX40 in SLNs from patients with primary cutaneous melanoma. Methods: Samples of peripheral blood lymphocytes and a section of 71 SLNs from 53 patients with clinically node negative melanoma were purified for CD4+ T cells, stained for OX40, and analyzed by flow cytometry. Results: The mean percentage of OX40 on CD4 T cells in the SLNs versus peripheral blood lymphocytes was related indirectly to the T stage of the primary tumor and was decreased in ulcerated primary tumors and positive sentinel nodes. Conclusions: The expression of OX40 on CD4+ T cells in SLNs draining primary melanomas decreased with more advanced tumor features (higher T stage, ulceration) and nodal involvement, suggesting that such tumors may have an immunosuppressive effect on the SLN microenvironment.

Original languageEnglish (US)
Pages (from-to)621-625
Number of pages5
JournalAmerican journal of surgery
Volume195
Issue number5
DOIs
StatePublished - May 1 2008

Keywords

  • Immunosuppressive environment
  • Melanoma
  • OX40
  • Sentinel lymph nodes

ASJC Scopus subject areas

  • Surgery

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