Ovine vascular endothelial growth factor: Nucleotide sequence and expression in fetal tissues

Cecilia Y. Cheung, Robert A. Brace

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

To examine the role of vascular endothelial growth factor (VEGF) in mediating angiogenesis and vascular permeability during fetal development, we determined the gene expression of VEGF in ovine fetal tissues. Further, we cloned and sequenced the ovine VEGF cDNA encoding VEGF164 from sheep placenta. VEGF protein was localized in epithelial cells of the placenta and fetal kidney, and in hepatocytes of the fetal liver. By Northern analysis, a major VEGF mRNA species of 3.7 kb was identified in all tissues examined, with abundance highest in the lung and lowest in the liver. The most prominent molecular form expressed in ovine fetal tissues appeared to be VEGF164 with low levels of expression of VEGF120 and VEGF188. Cloning and sequence analysis of the most abundant form of ovine VEGF cDNA in the placenta confirmed the prediction of a 164-amino acid peptide, with a putative N-terminal signal sequence of 26 amino acids. Comparison of the VEGF cDNA sequence among different species revealed that VEGF is highly conserved suggesting an important role in development.

Original languageEnglish (US)
Pages (from-to)11-22
Number of pages12
JournalGrowth Factors
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • DNA sequence
  • Fetus
  • Gene expression
  • Growth factor
  • Sheep

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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