Over-expression of osteopontin in non-small cell lung cancers: its clinical significance

Zhi Hu, Ting Xiao, Dong mei Lin, Shu ping Guo, Zi qiang Zhang, Xue bing Di, Shu jun Cheng, Yan ning Gao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: Data obtained from a differentially expressed cDNA library constructed previously in this laboratory demonstrated that the extracellular matrix molecule osteopontin (OPN) is one of most considerably over-expressed genes in non-small cell lung cancers (NSCLCs). The purpose of the present study was to explore the expression status of OPN in a large scale NSCLC tissue samples, and estimate its significance in progression of the malignant disease. METHODS: RT-PCR was performed with the tumor and adjacent normal tissues from 35 patients with NSCLC, at transcriptional levels of OPN. To determine the expression of OPN protein in the tumor tissues, immunohistochemical (IHC) staining was subsequently carried out on paraffin-embedded sections in tissue microarrays containing 662 samples derived from NSCLC cases. The correlation between the expression level of OPN and clinical characteristics was analyzed statistically. RESULTS: Comparing with the paired normal lung tissue, high level RNA of OPN was detected in 80.0% (28/35) of the NSCLC tumor tissues by RT-PCR, which confirmed the information obtained previously by our differentially expressed cDNA library. The results of IHC analysis showed that positively stained OPN protein was observed in 59.6% (331/555) of the tumor tissues, which was remarkably higher than that (25.2%, 27/107) detected in the normal control tissues (P <0.001). Among the NSCLCs investigated, over-expressed OPN was more frequently found in squamous cell carcinomas (SCCs) than in adenocarcinomas. A further analysis on SCCs demonstrated that the rate of over-expressed OPN was significantly different between the primary tumors with and without lymphatic metastases (68.6% vs. 49.7%, P = 0.001), but similar in the primary tumors and their corresponding metastases in lymph nodes (68.6% vs. 75.5%, P = 0.171). CONCLUSION: Expression of OPN protein is distinctly increased in NSCLCs, particularly in SCCs. OPN over-expression is considerably correlated with lymph node metastasis, increasing the risk of tumor metastasis (OR = 2.212). The resulting data suggest that OPN facilitates the progression of NSCLCs.

Original languageEnglish (US)
Pages (from-to)591-595
Number of pages5
JournalZhonghua zhong liu za zhi [Chinese journal of oncology]
Volume29
Issue number8
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

Osteopontin
Non-Small Cell Lung Carcinoma
Neoplasms
Squamous Cell Carcinoma
Neoplasm Metastasis
Gene Library
Lymph Nodes
Lymphatic Metastasis
Polymerase Chain Reaction
Proteins
Paraffin
Extracellular Matrix
Disease Progression
Adenocarcinoma

ASJC Scopus subject areas

  • Oncology

Cite this

Hu, Z., Xiao, T., Lin, D. M., Guo, S. P., Zhang, Z. Q., Di, X. B., ... Gao, Y. N. (2007). Over-expression of osteopontin in non-small cell lung cancers: its clinical significance. Zhonghua zhong liu za zhi [Chinese journal of oncology], 29(8), 591-595.

Over-expression of osteopontin in non-small cell lung cancers : its clinical significance. / Hu, Zhi; Xiao, Ting; Lin, Dong mei; Guo, Shu ping; Zhang, Zi qiang; Di, Xue bing; Cheng, Shu jun; Gao, Yan ning.

In: Zhonghua zhong liu za zhi [Chinese journal of oncology], Vol. 29, No. 8, 08.2007, p. 591-595.

Research output: Contribution to journalArticle

Hu, Z, Xiao, T, Lin, DM, Guo, SP, Zhang, ZQ, Di, XB, Cheng, SJ & Gao, YN 2007, 'Over-expression of osteopontin in non-small cell lung cancers: its clinical significance', Zhonghua zhong liu za zhi [Chinese journal of oncology], vol. 29, no. 8, pp. 591-595.
Hu, Zhi ; Xiao, Ting ; Lin, Dong mei ; Guo, Shu ping ; Zhang, Zi qiang ; Di, Xue bing ; Cheng, Shu jun ; Gao, Yan ning. / Over-expression of osteopontin in non-small cell lung cancers : its clinical significance. In: Zhonghua zhong liu za zhi [Chinese journal of oncology]. 2007 ; Vol. 29, No. 8. pp. 591-595.
@article{1aaa5bcf957f4b8095b02427ed47cf55,
title = "Over-expression of osteopontin in non-small cell lung cancers: its clinical significance",
abstract = "OBJECTIVE: Data obtained from a differentially expressed cDNA library constructed previously in this laboratory demonstrated that the extracellular matrix molecule osteopontin (OPN) is one of most considerably over-expressed genes in non-small cell lung cancers (NSCLCs). The purpose of the present study was to explore the expression status of OPN in a large scale NSCLC tissue samples, and estimate its significance in progression of the malignant disease. METHODS: RT-PCR was performed with the tumor and adjacent normal tissues from 35 patients with NSCLC, at transcriptional levels of OPN. To determine the expression of OPN protein in the tumor tissues, immunohistochemical (IHC) staining was subsequently carried out on paraffin-embedded sections in tissue microarrays containing 662 samples derived from NSCLC cases. The correlation between the expression level of OPN and clinical characteristics was analyzed statistically. RESULTS: Comparing with the paired normal lung tissue, high level RNA of OPN was detected in 80.0{\%} (28/35) of the NSCLC tumor tissues by RT-PCR, which confirmed the information obtained previously by our differentially expressed cDNA library. The results of IHC analysis showed that positively stained OPN protein was observed in 59.6{\%} (331/555) of the tumor tissues, which was remarkably higher than that (25.2{\%}, 27/107) detected in the normal control tissues (P <0.001). Among the NSCLCs investigated, over-expressed OPN was more frequently found in squamous cell carcinomas (SCCs) than in adenocarcinomas. A further analysis on SCCs demonstrated that the rate of over-expressed OPN was significantly different between the primary tumors with and without lymphatic metastases (68.6{\%} vs. 49.7{\%}, P = 0.001), but similar in the primary tumors and their corresponding metastases in lymph nodes (68.6{\%} vs. 75.5{\%}, P = 0.171). CONCLUSION: Expression of OPN protein is distinctly increased in NSCLCs, particularly in SCCs. OPN over-expression is considerably correlated with lymph node metastasis, increasing the risk of tumor metastasis (OR = 2.212). The resulting data suggest that OPN facilitates the progression of NSCLCs.",
author = "Zhi Hu and Ting Xiao and Lin, {Dong mei} and Guo, {Shu ping} and Zhang, {Zi qiang} and Di, {Xue bing} and Cheng, {Shu jun} and Gao, {Yan ning}",
year = "2007",
month = "8",
language = "English (US)",
volume = "29",
pages = "591--595",
journal = "Chinese Journal of Oncology",
issn = "0253-3766",
publisher = "Chinese Academy of Medical Sciences",
number = "8",

}

TY - JOUR

T1 - Over-expression of osteopontin in non-small cell lung cancers

T2 - its clinical significance

AU - Hu, Zhi

AU - Xiao, Ting

AU - Lin, Dong mei

AU - Guo, Shu ping

AU - Zhang, Zi qiang

AU - Di, Xue bing

AU - Cheng, Shu jun

AU - Gao, Yan ning

PY - 2007/8

Y1 - 2007/8

N2 - OBJECTIVE: Data obtained from a differentially expressed cDNA library constructed previously in this laboratory demonstrated that the extracellular matrix molecule osteopontin (OPN) is one of most considerably over-expressed genes in non-small cell lung cancers (NSCLCs). The purpose of the present study was to explore the expression status of OPN in a large scale NSCLC tissue samples, and estimate its significance in progression of the malignant disease. METHODS: RT-PCR was performed with the tumor and adjacent normal tissues from 35 patients with NSCLC, at transcriptional levels of OPN. To determine the expression of OPN protein in the tumor tissues, immunohistochemical (IHC) staining was subsequently carried out on paraffin-embedded sections in tissue microarrays containing 662 samples derived from NSCLC cases. The correlation between the expression level of OPN and clinical characteristics was analyzed statistically. RESULTS: Comparing with the paired normal lung tissue, high level RNA of OPN was detected in 80.0% (28/35) of the NSCLC tumor tissues by RT-PCR, which confirmed the information obtained previously by our differentially expressed cDNA library. The results of IHC analysis showed that positively stained OPN protein was observed in 59.6% (331/555) of the tumor tissues, which was remarkably higher than that (25.2%, 27/107) detected in the normal control tissues (P <0.001). Among the NSCLCs investigated, over-expressed OPN was more frequently found in squamous cell carcinomas (SCCs) than in adenocarcinomas. A further analysis on SCCs demonstrated that the rate of over-expressed OPN was significantly different between the primary tumors with and without lymphatic metastases (68.6% vs. 49.7%, P = 0.001), but similar in the primary tumors and their corresponding metastases in lymph nodes (68.6% vs. 75.5%, P = 0.171). CONCLUSION: Expression of OPN protein is distinctly increased in NSCLCs, particularly in SCCs. OPN over-expression is considerably correlated with lymph node metastasis, increasing the risk of tumor metastasis (OR = 2.212). The resulting data suggest that OPN facilitates the progression of NSCLCs.

AB - OBJECTIVE: Data obtained from a differentially expressed cDNA library constructed previously in this laboratory demonstrated that the extracellular matrix molecule osteopontin (OPN) is one of most considerably over-expressed genes in non-small cell lung cancers (NSCLCs). The purpose of the present study was to explore the expression status of OPN in a large scale NSCLC tissue samples, and estimate its significance in progression of the malignant disease. METHODS: RT-PCR was performed with the tumor and adjacent normal tissues from 35 patients with NSCLC, at transcriptional levels of OPN. To determine the expression of OPN protein in the tumor tissues, immunohistochemical (IHC) staining was subsequently carried out on paraffin-embedded sections in tissue microarrays containing 662 samples derived from NSCLC cases. The correlation between the expression level of OPN and clinical characteristics was analyzed statistically. RESULTS: Comparing with the paired normal lung tissue, high level RNA of OPN was detected in 80.0% (28/35) of the NSCLC tumor tissues by RT-PCR, which confirmed the information obtained previously by our differentially expressed cDNA library. The results of IHC analysis showed that positively stained OPN protein was observed in 59.6% (331/555) of the tumor tissues, which was remarkably higher than that (25.2%, 27/107) detected in the normal control tissues (P <0.001). Among the NSCLCs investigated, over-expressed OPN was more frequently found in squamous cell carcinomas (SCCs) than in adenocarcinomas. A further analysis on SCCs demonstrated that the rate of over-expressed OPN was significantly different between the primary tumors with and without lymphatic metastases (68.6% vs. 49.7%, P = 0.001), but similar in the primary tumors and their corresponding metastases in lymph nodes (68.6% vs. 75.5%, P = 0.171). CONCLUSION: Expression of OPN protein is distinctly increased in NSCLCs, particularly in SCCs. OPN over-expression is considerably correlated with lymph node metastasis, increasing the risk of tumor metastasis (OR = 2.212). The resulting data suggest that OPN facilitates the progression of NSCLCs.

UR - http://www.scopus.com/inward/record.url?scp=49549084319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49549084319&partnerID=8YFLogxK

M3 - Article

C2 - 18210878

AN - SCOPUS:49549084319

VL - 29

SP - 591

EP - 595

JO - Chinese Journal of Oncology

JF - Chinese Journal of Oncology

SN - 0253-3766

IS - 8

ER -