Ovarian steroids increase PSD-95 expression and dendritic spines in the dorsal raphe of ovariectomized macaques

Heidi M. Rivera, Cynthia Bethea

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Estradiol (E) and progesterone (P) promote spinogenesis in several brain areas. Intracellular signaling cascades that promote spinogenesis involve RhoGTPases, glutamate signaling and synapse assembly. We found that in serotonin neurons, E ± P administration increases (a) gene and protein expression of RhoGTPases, (b) gene expression of glutamate receptors, and (c) gene expression of pivotal synapse assembly proteins. Therefore, in this study we determined whether structural changes in dendritic spines in the dorsal raphe follow the observed changes in gene and protein expression. Dendritic spines were examined with immunogold silver staining of a spine marker protein, postsynaptic density-95 (PSD-95) and with Golgi staining. In the PSD-95 study, adult Ovx monkeys received placebo, E, P, or E + P for 1 month (n = 3/group). Sections were immunostained for PSD-95 and the number of PSD-95-positive puncta was determined with stereology. E, P, and E + P treatment significantly increased the total number of PSD-95-positive puncta (ANOVA, P = 0.04). In the golgi study, adult Ovx monkeys received placebo, E or E + P for 1 month (n = 3-4) and the midbrain was golgi-stained. A total of 80 neurons were analyzed with Neurolucida software. There was a significant difference in spine density that depended on branch order (two-way ANOVA). E + P treatment significantly increased spine density in higher-order (3°-5°) dendritic branches relative to Ovx group (Bonferroni, P <0.05). In summary, E + P leads to the elaboration of dendritic spines on dorsal raphe neurons. The ability of E to induce PSD-95, but not actual spines, suggests either a sampling or time lag issue. Increased spinogenesis on serotonin dendrites would facilitate excitatory glutamatergic input and, in turn, increase serotonin neurotransmission throughout the brain.

    Original languageEnglish (US)
    Pages (from-to)897-908
    Number of pages12
    JournalSynapse
    Volume67
    Issue number12
    DOIs
    StatePublished - Dec 2013

    Fingerprint

    Post-Synaptic Density
    Dendritic Spines
    Macaca
    Steroids
    Spine
    Gene Expression
    Serotonin
    Neurons
    Synapses
    Haplorhini
    Analysis of Variance
    Placebos
    Silver Staining
    Proteins
    Glutamate Receptors
    Brain
    Dendrites
    Mesencephalon
    Synaptic Transmission
    Progesterone

    Keywords

    • Dendritic spines
    • Estradiol
    • Golgi
    • Progesterone
    • Serotonin

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience

    Cite this

    Ovarian steroids increase PSD-95 expression and dendritic spines in the dorsal raphe of ovariectomized macaques. / Rivera, Heidi M.; Bethea, Cynthia.

    In: Synapse, Vol. 67, No. 12, 12.2013, p. 897-908.

    Research output: Contribution to journalArticle

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