Ovarian steroids decrease DNA fragmentation in the serotonin neurons of non-injured rhesus macaques

F. B. Lima, Cynthia Bethea

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    We previously found that ovarian steroids promote neuroprotection in serotonin neurons by decreasing the expression of pro-apoptotic genes and proteins in the dorsal raphe nucleus of rhesus macaques, even in the absence of overt injury. In this study, we questioned whether these actions would lead to a reduction in DNA fragmentation in serotonin neurons. Ovariectomized (OVX) rhesus monkeys were implanted with silastic capsules that were empty (placebo) or containing estradiol (E), progesterone (P) or estradiol and progesterone (EP) for 1 month. In all animals, eight levels of the dorsal raphe nucleus in a rostral-to-caudal direction were immunostained using the terminal deoxynucleotidyl transferase nick end labeling (TUNEL) method. Two staining patterns were observed, which are referred to as type I, with complete dark staining of the nucleus, and type II, with peripheral staining in the perinuclear area. A montage of the dorsal raphe was created at each level with a Marianas Stereology Microscope and Slidebook 4.2, and the TUNEL-positive cells were counted. In direct comparison with OVX animals, P treatment and EP treatment significantly reduced the total number of TUNEL-positive cells (Mann-Whitney test, both treatments P=0.04) and EP treatment reduced the number of TUNEL-positive cells per mm3 (Mann-Whitney test, P=0.04). Double immunocytochemistry for TUNEL and tryptophan hydroxylase (TPH) indicated that DNA fragmentation was prominent in serotonin neurons. These data suggest that in the absence of ovarian steroids, a cascade of gene and protein expression leads to an increase in DNA fragmentation in serotonin neurons. Conversely, ovarian steroids have a neuroprotective role in the non-injured brain and prevent DNA fragmentation and cell death in serotonin neurons of nonhuman primates.

    Original languageEnglish (US)
    Pages (from-to)657-668
    Number of pages12
    JournalMolecular Psychiatry
    Volume15
    Issue number6
    DOIs
    StatePublished - Jun 2010

    Fingerprint

    DNA Nucleotidylexotransferase
    DNA Fragmentation
    Macaca mulatta
    Serotonin
    Steroids
    Progesterone
    Estradiol
    Neurons
    Staining and Labeling
    Tryptophan Hydroxylase
    Apoptosis Regulatory Proteins
    Therapeutics
    Primates
    Capsules
    Proteins
    Cell Death
    Immunohistochemistry
    Placebos
    Gene Expression
    Wounds and Injuries

    Keywords

    • Apoptosis
    • Dorsal raphe nucleus
    • Estrogen
    • Progesterone
    • Serotonin
    • TUNEL

    ASJC Scopus subject areas

    • Molecular Biology
    • Psychiatry and Mental health
    • Cellular and Molecular Neuroscience

    Cite this

    Ovarian steroids decrease DNA fragmentation in the serotonin neurons of non-injured rhesus macaques. / Lima, F. B.; Bethea, Cynthia.

    In: Molecular Psychiatry, Vol. 15, No. 6, 06.2010, p. 657-668.

    Research output: Contribution to journalArticle

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