Ovarian innervation develops before initiation of folliculogenesis in the rat

Sasha Malamed, Jean A. Gibney, Sergio Ojeda

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Sympathetic neurotransmitters have been shown to be present in the ovary of the rat during early postnatal development and to affect steroidogenesis before the ovary becomes responsive to gonadotropins, and before the first primordial follicles are formed. This study was undertaken to determine if development of the ovarian innervation is an event that antedates the initiation of folliculogenesis in the rat, Rattus norvegicus. Serial sections of postnatal ovaries revealed a negligible frequency of follicles 24 h after birth (about 1 primordial follicle per ovary). Twelve hours later there were about 500 follicles per ovary, a number that more than doubled to about 1300 during the subsequent 12 h, indicating that an explosive period of follicular differentiation occurs between the end of postnatal days 1 and 2. Electron microscopy demonstrated that before birth the ovaries are already innervated by fibers containing clear and dense-core vesicles. Immunohistochemistry performed on either fetal (day 19) or newborn (less than 15h after birth) ovaries showed the presence of catecholaminergic nerves, identified by their content of immunoreactive tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis. While some of these fibers innervate blood vessels, others are associated with primordial ovarian cells, thereby suggesting their participation in non-vascular functions. Since prefollicular ovaries are insensitive to gonadotropins, the results suggest that the developing ovary becomes subjected to direct neurogenic influences before it acquires responsiveness to gonadotropins.

    Original languageEnglish (US)
    Pages (from-to)87-93
    Number of pages7
    JournalCell & Tissue Research
    Volume270
    Issue number1
    DOIs
    StatePublished - Oct 1992

    Fingerprint

    Gonadotropins
    Rats
    Ovary
    Fibers
    Biosynthesis
    Blood vessels
    Tyrosine 3-Monooxygenase
    Electron microscopy
    Catecholamines
    Neurotransmitter Agents
    Parturition
    Enzymes
    Secretory Vesicles
    Blood Vessels
    Electron Microscopy
    Immunohistochemistry

    Keywords

    • Development
    • Electron microscopy
    • Folliculogenesis
    • Immunohistochemistry
    • Ovarian nerves
    • Rat (Sprague Dawley)
    • Tyrosine hydroxylase

    ASJC Scopus subject areas

    • Anatomy
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    Ovarian innervation develops before initiation of folliculogenesis in the rat. / Malamed, Sasha; Gibney, Jean A.; Ojeda, Sergio.

    In: Cell & Tissue Research, Vol. 270, No. 1, 10.1992, p. 87-93.

    Research output: Contribution to journalArticle

    Malamed, Sasha ; Gibney, Jean A. ; Ojeda, Sergio. / Ovarian innervation develops before initiation of folliculogenesis in the rat. In: Cell & Tissue Research. 1992 ; Vol. 270, No. 1. pp. 87-93.
    @article{eda2bc91cc6742b9b9a1ba1537b056db,
    title = "Ovarian innervation develops before initiation of folliculogenesis in the rat",
    abstract = "Sympathetic neurotransmitters have been shown to be present in the ovary of the rat during early postnatal development and to affect steroidogenesis before the ovary becomes responsive to gonadotropins, and before the first primordial follicles are formed. This study was undertaken to determine if development of the ovarian innervation is an event that antedates the initiation of folliculogenesis in the rat, Rattus norvegicus. Serial sections of postnatal ovaries revealed a negligible frequency of follicles 24 h after birth (about 1 primordial follicle per ovary). Twelve hours later there were about 500 follicles per ovary, a number that more than doubled to about 1300 during the subsequent 12 h, indicating that an explosive period of follicular differentiation occurs between the end of postnatal days 1 and 2. Electron microscopy demonstrated that before birth the ovaries are already innervated by fibers containing clear and dense-core vesicles. Immunohistochemistry performed on either fetal (day 19) or newborn (less than 15h after birth) ovaries showed the presence of catecholaminergic nerves, identified by their content of immunoreactive tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis. While some of these fibers innervate blood vessels, others are associated with primordial ovarian cells, thereby suggesting their participation in non-vascular functions. Since prefollicular ovaries are insensitive to gonadotropins, the results suggest that the developing ovary becomes subjected to direct neurogenic influences before it acquires responsiveness to gonadotropins.",
    keywords = "Development, Electron microscopy, Folliculogenesis, Immunohistochemistry, Ovarian nerves, Rat (Sprague Dawley), Tyrosine hydroxylase",
    author = "Sasha Malamed and Gibney, {Jean A.} and Sergio Ojeda",
    year = "1992",
    month = "10",
    doi = "10.1007/BF00381883",
    language = "English (US)",
    volume = "270",
    pages = "87--93",
    journal = "Cell and Tissue Research",
    issn = "0302-766X",
    publisher = "Springer Verlag",
    number = "1",

    }

    TY - JOUR

    T1 - Ovarian innervation develops before initiation of folliculogenesis in the rat

    AU - Malamed, Sasha

    AU - Gibney, Jean A.

    AU - Ojeda, Sergio

    PY - 1992/10

    Y1 - 1992/10

    N2 - Sympathetic neurotransmitters have been shown to be present in the ovary of the rat during early postnatal development and to affect steroidogenesis before the ovary becomes responsive to gonadotropins, and before the first primordial follicles are formed. This study was undertaken to determine if development of the ovarian innervation is an event that antedates the initiation of folliculogenesis in the rat, Rattus norvegicus. Serial sections of postnatal ovaries revealed a negligible frequency of follicles 24 h after birth (about 1 primordial follicle per ovary). Twelve hours later there were about 500 follicles per ovary, a number that more than doubled to about 1300 during the subsequent 12 h, indicating that an explosive period of follicular differentiation occurs between the end of postnatal days 1 and 2. Electron microscopy demonstrated that before birth the ovaries are already innervated by fibers containing clear and dense-core vesicles. Immunohistochemistry performed on either fetal (day 19) or newborn (less than 15h after birth) ovaries showed the presence of catecholaminergic nerves, identified by their content of immunoreactive tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis. While some of these fibers innervate blood vessels, others are associated with primordial ovarian cells, thereby suggesting their participation in non-vascular functions. Since prefollicular ovaries are insensitive to gonadotropins, the results suggest that the developing ovary becomes subjected to direct neurogenic influences before it acquires responsiveness to gonadotropins.

    AB - Sympathetic neurotransmitters have been shown to be present in the ovary of the rat during early postnatal development and to affect steroidogenesis before the ovary becomes responsive to gonadotropins, and before the first primordial follicles are formed. This study was undertaken to determine if development of the ovarian innervation is an event that antedates the initiation of folliculogenesis in the rat, Rattus norvegicus. Serial sections of postnatal ovaries revealed a negligible frequency of follicles 24 h after birth (about 1 primordial follicle per ovary). Twelve hours later there were about 500 follicles per ovary, a number that more than doubled to about 1300 during the subsequent 12 h, indicating that an explosive period of follicular differentiation occurs between the end of postnatal days 1 and 2. Electron microscopy demonstrated that before birth the ovaries are already innervated by fibers containing clear and dense-core vesicles. Immunohistochemistry performed on either fetal (day 19) or newborn (less than 15h after birth) ovaries showed the presence of catecholaminergic nerves, identified by their content of immunoreactive tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis. While some of these fibers innervate blood vessels, others are associated with primordial ovarian cells, thereby suggesting their participation in non-vascular functions. Since prefollicular ovaries are insensitive to gonadotropins, the results suggest that the developing ovary becomes subjected to direct neurogenic influences before it acquires responsiveness to gonadotropins.

    KW - Development

    KW - Electron microscopy

    KW - Folliculogenesis

    KW - Immunohistochemistry

    KW - Ovarian nerves

    KW - Rat (Sprague Dawley)

    KW - Tyrosine hydroxylase

    UR - http://www.scopus.com/inward/record.url?scp=0026756307&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026756307&partnerID=8YFLogxK

    U2 - 10.1007/BF00381883

    DO - 10.1007/BF00381883

    M3 - Article

    C2 - 1358455

    AN - SCOPUS:0026756307

    VL - 270

    SP - 87

    EP - 93

    JO - Cell and Tissue Research

    JF - Cell and Tissue Research

    SN - 0302-766X

    IS - 1

    ER -