The cell envelope of S. sonnei phase I, phase II and R-form was fractionated into outer and cytoplasmic membrane components by means of sucrose density gradient centrifugation. The protein composition of the outer membrane has been analyzed by SDS-polyacrylamide gel electrophoresis. The outer membrane preparations contained 15-17 proteins. The major proteins of the outer membrane were of apparent molecular weights: 27,000, 28,000 and 31, 000. Their amount varied depending on the structural defects of lipopolysaccharide.
|Original language||English (US)|
|Number of pages||6|
|Journal||Archivum immunologiae et therapiae experimentalis|
|State||Published - Dec 1 1980|
ASJC Scopus subject areas
- Immunology and Allergy