OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function

Yu Chi Juang, Marie Claude Landry, Mario Sanches, Vinayak Vittal, Charles C.Y. Leung, Derek F. Ceccarelli, Abigail Rachele F. Mateo, Jonathan Pruneda, Daniel Y.L. Mao, Rachel K. Szilard, Stephen Orlicky, Meagan Munro, Peter S. Brzovic, Rachel E. Klevit, Frank Sicheri, Daniel Durocher

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Ubiquitylation entails the concerted action of E1, E2, and E3 enzymes. We recently reported that OTUB1, a deubiquitylase, inhibits the DNA damage response independently of its isopeptidase activity. OTUB1 does so by blocking ubiquitin transfer by UBC13, the cognate E2 enzyme for RNF168. OTUB1 also inhibits E2s of the UBE2D and UBE2E families. Herewe elucidate the structural mechanism by which OTUB1 binds E2s to inhibit ubiquitin transfer. OTUB1 recognizes ubiquitin-charged E2s through contacts with both donor ubiquitin and the E2 enzyme. Surprisingly, free ubiquitin associates with the canonical distal ubiquitin-binding site on OTUB1 to promote formation of the inhibited E2 complex. Lys48 of donor ubiquitin lies near the OTUB1 catalytic site and the C terminus of free ubiquitin, a configuration that mimics the products of Lys48-linked ubiquitin chain cleavage. OTUB1 therefore co-opts Lys48-linked ubiquitin chain recognition to suppress ubiquitin conjugation and the DNA damage response.

Original languageEnglish (US)
Pages (from-to)384-397
Number of pages14
JournalMolecular Cell
Volume45
Issue number3
DOIs
StatePublished - Feb 10 2012
Externally publishedYes

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Ubiquitin
Enzymes
DNA Damage
Ubiquitination
Catalytic Domain
Binding Sites

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Juang, Y. C., Landry, M. C., Sanches, M., Vittal, V., Leung, C. C. Y., Ceccarelli, D. F., ... Durocher, D. (2012). OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function. Molecular Cell, 45(3), 384-397. https://doi.org/10.1016/j.molcel.2012.01.011

OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function. / Juang, Yu Chi; Landry, Marie Claude; Sanches, Mario; Vittal, Vinayak; Leung, Charles C.Y.; Ceccarelli, Derek F.; Mateo, Abigail Rachele F.; Pruneda, Jonathan; Mao, Daniel Y.L.; Szilard, Rachel K.; Orlicky, Stephen; Munro, Meagan; Brzovic, Peter S.; Klevit, Rachel E.; Sicheri, Frank; Durocher, Daniel.

In: Molecular Cell, Vol. 45, No. 3, 10.02.2012, p. 384-397.

Research output: Contribution to journalArticle

Juang, YC, Landry, MC, Sanches, M, Vittal, V, Leung, CCY, Ceccarelli, DF, Mateo, ARF, Pruneda, J, Mao, DYL, Szilard, RK, Orlicky, S, Munro, M, Brzovic, PS, Klevit, RE, Sicheri, F & Durocher, D 2012, 'OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function', Molecular Cell, vol. 45, no. 3, pp. 384-397. https://doi.org/10.1016/j.molcel.2012.01.011
Juang YC, Landry MC, Sanches M, Vittal V, Leung CCY, Ceccarelli DF et al. OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function. Molecular Cell. 2012 Feb 10;45(3):384-397. https://doi.org/10.1016/j.molcel.2012.01.011
Juang, Yu Chi ; Landry, Marie Claude ; Sanches, Mario ; Vittal, Vinayak ; Leung, Charles C.Y. ; Ceccarelli, Derek F. ; Mateo, Abigail Rachele F. ; Pruneda, Jonathan ; Mao, Daniel Y.L. ; Szilard, Rachel K. ; Orlicky, Stephen ; Munro, Meagan ; Brzovic, Peter S. ; Klevit, Rachel E. ; Sicheri, Frank ; Durocher, Daniel. / OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function. In: Molecular Cell. 2012 ; Vol. 45, No. 3. pp. 384-397.
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