Otoprotective effects of dexamethasone in the management of pneumococcal meningitis: An animal study

Harold H. Kim, John Addison, Eul Suh, Dennis Trune, Claus Peter Richter

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

OBJECTIVE: To determine whether treating pneumococcal meningitis with a combined antibiotic and steroid regime will prevent cochlear damage, a common pneumococcal meningitis side effect. STUDY DESIGN: Prospective animal study. METHODS: Gerbils were randomly assigned to three experimental groups. Animals in group 1 received intrathecal saline injections. Animals in groups 2 and 3 received intrathecal injections of Streptococcus pneumoniae to induce meningitis. Group 2 was treated for 7 days with intraperitoneal penicillin injections (48,000 units). Animals from group 3 received intraperitoneal dexamethasone (0.5 mg/kg) injections for 4 days in addition to 7 days of intraperitoneal penicillin. Three months after the meningitis was induced, the animals' cochlear functions were determined using auditory brainstem responses (ABRs). After measuring cochlear function, the animals were sacrificed for cochlear histopathology. Spiral ganglion cell densities at Rosenthal's canal were determined. RESULTS: ABR thresholds were significantly elevated in animals from group 2 when compared with the animals in groups 1 and 3 (P <.05). ABR thresholds for animals from group 3 and group 1 were similar (P > .05). Damage of cochlear structures was detected in animals from group 2. The degree of the damage varied: one animal in group 2 had no identifiable hair cells and pillarcells and showed damage of the tectorial membrane. Spiral ganglion density in the basal turn was significantly less inanimals from group 2 when compared withcontrols (P <.05). Although spiral ganglion cell density was less in the dexamethasone-treated group (group 3) whencompared with group 1 (control group), but greater than observed in animals treated with antibiotics only (group 2), the differences were statistically not significant (P > .5). Nuclear diameters of the spiral ganglion cells decreased on average from 7.24 ± 0.48 μm (group 1) to 6.28 ± 0.76 μm (group 3, animals that received dexamethasone) to 5.57 ± 0.82 μm (group 2, animals that received antibiotics only). Differences were significant (P <.05). Differences in stria vascularis thickness were not significant among the animals. CONCLUSION: Dexamethasone has a protective effect on the cochlea when given together with antibiotics in the treatment of pneumococcal meningitis.

Original languageEnglish (US)
Pages (from-to)1209-1215
Number of pages7
JournalLaryngoscope
Volume117
Issue number7
DOIs
StatePublished - Jul 2007

Fingerprint

Pneumococcal Meningitis
Dexamethasone
Cochlea
Spiral Ganglion
Spinal Injections
Brain Stem Auditory Evoked Potentials
Anti-Bacterial Agents
Meningitis
Penicillins
Tectorial Membrane
Stria Vascularis
Gerbillinae
Streptococcus pneumoniae
Intraperitoneal Injections

Keywords

  • Dexamethasone
  • Meningitis
  • Pneumococcus
  • Steroid
  • Streptococcus pneumonia

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Otoprotective effects of dexamethasone in the management of pneumococcal meningitis : An animal study. / Kim, Harold H.; Addison, John; Suh, Eul; Trune, Dennis; Richter, Claus Peter.

In: Laryngoscope, Vol. 117, No. 7, 07.2007, p. 1209-1215.

Research output: Contribution to journalArticle

Kim, Harold H. ; Addison, John ; Suh, Eul ; Trune, Dennis ; Richter, Claus Peter. / Otoprotective effects of dexamethasone in the management of pneumococcal meningitis : An animal study. In: Laryngoscope. 2007 ; Vol. 117, No. 7. pp. 1209-1215.
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abstract = "OBJECTIVE: To determine whether treating pneumococcal meningitis with a combined antibiotic and steroid regime will prevent cochlear damage, a common pneumococcal meningitis side effect. STUDY DESIGN: Prospective animal study. METHODS: Gerbils were randomly assigned to three experimental groups. Animals in group 1 received intrathecal saline injections. Animals in groups 2 and 3 received intrathecal injections of Streptococcus pneumoniae to induce meningitis. Group 2 was treated for 7 days with intraperitoneal penicillin injections (48,000 units). Animals from group 3 received intraperitoneal dexamethasone (0.5 mg/kg) injections for 4 days in addition to 7 days of intraperitoneal penicillin. Three months after the meningitis was induced, the animals' cochlear functions were determined using auditory brainstem responses (ABRs). After measuring cochlear function, the animals were sacrificed for cochlear histopathology. Spiral ganglion cell densities at Rosenthal's canal were determined. RESULTS: ABR thresholds were significantly elevated in animals from group 2 when compared with the animals in groups 1 and 3 (P <.05). ABR thresholds for animals from group 3 and group 1 were similar (P > .05). Damage of cochlear structures was detected in animals from group 2. The degree of the damage varied: one animal in group 2 had no identifiable hair cells and pillarcells and showed damage of the tectorial membrane. Spiral ganglion density in the basal turn was significantly less inanimals from group 2 when compared withcontrols (P <.05). Although spiral ganglion cell density was less in the dexamethasone-treated group (group 3) whencompared with group 1 (control group), but greater than observed in animals treated with antibiotics only (group 2), the differences were statistically not significant (P > .5). Nuclear diameters of the spiral ganglion cells decreased on average from 7.24 ± 0.48 μm (group 1) to 6.28 ± 0.76 μm (group 3, animals that received dexamethasone) to 5.57 ± 0.82 μm (group 2, animals that received antibiotics only). Differences were significant (P <.05). Differences in stria vascularis thickness were not significant among the animals. CONCLUSION: Dexamethasone has a protective effect on the cochlea when given together with antibiotics in the treatment of pneumococcal meningitis.",
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