Otitis Media Impacts Hundreds of Mouse Middle and Inner Ear Genes

Carol Macarthur, Fran Hausman, J. Beth Kempton, Dongseok Choi, Dennis Trune

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Objective:Otitis media is known to alter expression of cytokine and other genes in the mouse middle ear and inner ear. However, whole mouse genome studies of gene expression in otitis media have not previously been undertaken. Ninety-nine percent of mouse genes are shared in the human, so these studies are relevant to the human condition.Methods:To assess inflammation-driven processes in the mouse ear, gene chip analyses were conducted on mice treated with trans-tympanic heat-killed Hemophilus influenza using untreated mice as controls. Middle and inner ear tissues were separately harvested at 6 hours, RNA extracted, and samples for each treatment processed on the Affymetrix 430 2.0 Gene Chip for expression of its 34,000 genes.Results:Statistical analysis of gene expression compared to control mice showed significant alteration of gene expression in 2,355 genes, 11% of the genes tested and 8% of the mouse genome. Significant middle and inner ear upregulation (fold change >1.5, p

Original languageEnglish (US)
Article numbere75213
JournalPLoS One
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 4 2013

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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