Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis

Sandra M. Ruzal, Claudia Lopez, Emilio Rivas, Carmen Sánchez-Rivas

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In Bacillus subtilis, osmotolerance is a stationary phase-dependent, adaptive response inhibiting sporulation and sharing common regulators with this process. The extent of this inhibition was determined by measuring transcription activity of promoter lacZ fusions to early sigma genes (spoIIG and spoIIA coding for precursors of oσ(E) and σ(F)) and to reporters of them (spoIID, spoIIQ and spoIIIG), in the absence and presence of 0.6 M or 1 M NaCl. The transcription activity of these sigma precursors, normally occurring at the onset of the stationary phase, was reduced to 30-50% of their maximal expression in hyperosmotic conditions; expression of genes under their control was, however, more inhibited (<10%). Therefore, sporulation was blocked at the sigma σ(E) and σ(F) activation steps. This assumption was confirmed by electron microscopic examinations of hyperosmotic cultures, which presented asymmetric septa characteristic of stage II mutants. Discussion was focused on the particular composition and/or structure of membranes during hyperosmotic growth and their involvement in the arrest of sporulation.

Original languageEnglish (US)
Pages (from-to)75-79
Number of pages5
JournalCurrent Microbiology
Volume36
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

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Sigma Factor
Bacillus subtilis
Electrons
Gene Expression
Membranes
Growth
Genes

ASJC Scopus subject areas

  • Microbiology

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Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis. / Ruzal, Sandra M.; Lopez, Claudia; Rivas, Emilio; Sánchez-Rivas, Carmen.

In: Current Microbiology, Vol. 36, No. 2, 1998, p. 75-79.

Research output: Contribution to journalArticle

Ruzal, Sandra M. ; Lopez, Claudia ; Rivas, Emilio ; Sánchez-Rivas, Carmen. / Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis. In: Current Microbiology. 1998 ; Vol. 36, No. 2. pp. 75-79.
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