Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus

Denise I. Quigley, John McDougall, Tristan Darland, Ge Zhang, Oline Ronnekliev, David K. Grandy, Richard G. Allen

    Research output: Contribution to journalArticlepeer-review

    28 Scopus citations


    In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed- phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co- eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.

    Original languageEnglish (US)
    Pages (from-to)133-139
    Number of pages7
    Issue number1
    StatePublished - 1998


    • Hypothalamus
    • Neuropeptides
    • Orphanin FQ
    • Processing

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Cellular and Molecular Neuroscience


    Dive into the research topics of 'Orphanin FQ is the major OFQ<sup>1-17</sup>-containing peptide produced in the rodent and monkey hypothalamus'. Together they form a unique fingerprint.

    Cite this