Origin and ontogeny of mammalian ovarian neurons

W. Les Dees, J. K. Hiney, N. H. McArthur, G. A. Johnson, Gregory Dissen, Sergio Ojeda

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Mammalian ovaries contain sympathetic neurons expressing the low affinity neurotropin receptor (p75NTR). To date neither the role these neurons might play in ovarian physiology nor their embryological origin is known. Immunohistochemistry was used to detect postnatal changes in distribution and number of both p75NTR-positive and tyrosine hydroxylase-positive neurons in rhesus monkey ovaries. Pig fetuses were used to map the pathway of ovarian neuronal migration during embryonic development. Antiserum to p75 NTR revealed the presence of isolated neurons and neurons clustered into ganglia in 2-month-old monkey ovaries. After 8 months, the neurons exhibited well-developed processes, and other than being more extensively interlaced, the localization and morphology did not change after 2 yr of age. Total number of p75NTR-positive neurons present decreased gradually between 2 months and 12 yr of age and declined markedly with reproductive aging. Conversely, the subpopulation of neurons immunoreactive to anti-tyrosine hydroxylase increased significantly at puberty and then declined with the loss of reproductive capacity. By d 21 of fetal life in the pig, p75NTR neurons had migrated medially from the neural crest to form the paraaortic autonomic ganglia. Some neurons migrated ventrally from the ganglia and then continued ventrolaterally to enter the genital ridge. By d 27, neurons had entered the developing ovary, and by d 35, the migration was complete with neurons demonstrating immunoreactivity to NeuN, a neuron-specific marker. Results demonstrate that p75NTR-expressing ovarian neurons originate from the neural crest and that a catecholaminergic subset is associated with pubertal maturation of the ovary and subsequent reproductive function.

    Original languageEnglish (US)
    Pages (from-to)3789-3796
    Number of pages8
    JournalEndocrinology
    Volume147
    Issue number8
    DOIs
    StatePublished - 2006

    Fingerprint

    Neurons
    Ovary
    Neural Crest
    Tyrosine 3-Monooxygenase
    Ganglia
    Swine
    Autonomic Ganglia
    Puberty
    Macaca mulatta
    Embryonic Development
    Haplorhini
    Immune Sera
    Fetus
    Immunohistochemistry

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Les Dees, W., Hiney, J. K., McArthur, N. H., Johnson, G. A., Dissen, G., & Ojeda, S. (2006). Origin and ontogeny of mammalian ovarian neurons. Endocrinology, 147(8), 3789-3796. https://doi.org/10.1210/en.2006-0394

    Origin and ontogeny of mammalian ovarian neurons. / Les Dees, W.; Hiney, J. K.; McArthur, N. H.; Johnson, G. A.; Dissen, Gregory; Ojeda, Sergio.

    In: Endocrinology, Vol. 147, No. 8, 2006, p. 3789-3796.

    Research output: Contribution to journalArticle

    Les Dees, W, Hiney, JK, McArthur, NH, Johnson, GA, Dissen, G & Ojeda, S 2006, 'Origin and ontogeny of mammalian ovarian neurons', Endocrinology, vol. 147, no. 8, pp. 3789-3796. https://doi.org/10.1210/en.2006-0394
    Les Dees W, Hiney JK, McArthur NH, Johnson GA, Dissen G, Ojeda S. Origin and ontogeny of mammalian ovarian neurons. Endocrinology. 2006;147(8):3789-3796. https://doi.org/10.1210/en.2006-0394
    Les Dees, W. ; Hiney, J. K. ; McArthur, N. H. ; Johnson, G. A. ; Dissen, Gregory ; Ojeda, Sergio. / Origin and ontogeny of mammalian ovarian neurons. In: Endocrinology. 2006 ; Vol. 147, No. 8. pp. 3789-3796.
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