Organization of the core structure of the postsynaptic density

Xiaobing Chen, Christine Winters, Rita Azzam, Xiang Li, James Galbraith, Richard D. Leapman, Thomas S. Reese

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Much is known about the composition and function of the postsynaptic density (PSD), but less is known about its molecular organization. We use EM tomography to delineate the organization of PSDs at glutamatergic synapses in rat hippocampal cultures. The core of the PSD is dominated by vertically oriented filaments, and ImmunoGold labeling shows that PSD-95 is a component of these filaments. Vertical filaments contact two types of transmembrane structures whose sizes and positions match those of glutamate receptors and intermesh with two types of horizontally oriented filaments lying 10-20 nm from the postsynaptic membrane. The longer horizontal filaments link adjacent NMDAR-type structures, whereas the smaller filaments link both NMDA- and AMPAR-type structures. The orthogonal, interlinked scaffold of filaments at the core of the PSD provides a structural basis for understanding dynamic aspects of postsynaptic function.

Original languageEnglish (US)
Pages (from-to)4453-4458
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number11
DOIs
StatePublished - Mar 18 2008
Externally publishedYes

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Post-Synaptic Density
Electron Microscope Tomography
Glutamate Receptors
N-Methylaspartate
Synapses
Membranes

Keywords

  • EM tomography
  • High-pressure freezing
  • Hippocampal neuron
  • PSD-95

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Organization of the core structure of the postsynaptic density. / Chen, Xiaobing; Winters, Christine; Azzam, Rita; Li, Xiang; Galbraith, James; Leapman, Richard D.; Reese, Thomas S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 11, 18.03.2008, p. 4453-4458.

Research output: Contribution to journalArticle

Chen, Xiaobing ; Winters, Christine ; Azzam, Rita ; Li, Xiang ; Galbraith, James ; Leapman, Richard D. ; Reese, Thomas S. / Organization of the core structure of the postsynaptic density. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 11. pp. 4453-4458.
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