Organization of cortical and thalamic input to pyramidal neurons in mouse motor cortex

Bryan M. Hooks, Tianyi Mao, Diego A. Gutnisky, Naoki Yamawaki, Karel Svoboda, Gordon M G Shepherd

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Determining how long-range synaptic inputs engage pyramidal neurons in primary motor cortex (M1) is important for understanding circuit mechanisms involved in regulating movement. We used channelrhodopsin-2-assisted circuit mapping to characterize the longrange excitatory synaptic connections made by multiple cortical and thalamic areas onto pyramidal neurons in mouse vibrissal motor cortex (vM1). Each projection innervated vM1 pyramidal neurons with a unique laminar profile. Collectively, the profiles for different sources of input partially overlapped and spanned all cortical layers. Specifically, orbital cortex (OC) inputs primarily targeted neurons in L6. Secondary motor cortex (M2) inputs excited neurons mainly in L5B, including pyramidal tract neurons. In contrast, thalamocortical inputs from anterior motor-related thalamic regions, including VA/VL (ventral anterior thalamic nucleus/ventrolateral thalamic nucleus), targeted neurons in L2/3 through L5B, but avoided L6. Inputs from posterior sensory-related thalamic areas, including POm (posterior thalamic nuclear group), targeted neurons only in the upper layers (L2/3 and L5A), similar to inputs from somatosensory (barrel) cortex. Our results show that long-range excitatory inputs target vM1 pyramidal neurons in a layer-specific manner. Inputs from sensory-related cortical and thalamic areas preferentially target the upper-layer pyramidal neurons in vM1. In contrast, inputs from OC and M2, areas associated with volitional and cognitive aspects of movements, bypass local circuitry and have direct monosynaptic access to neurons projecting to brainstem and thalamus.

Original languageEnglish (US)
Pages (from-to)748-760
Number of pages13
JournalJournal of Neuroscience
Volume33
Issue number2
DOIs
StatePublished - Jan 9 2013

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Pyramidal Cells
Motor Cortex
Neurons
Ventral Thalamic Nuclei
Prefrontal Cortex
Posterior Thalamic Nuclei
Pyramidal Tracts
Somatosensory Cortex
Thalamus
Brain Stem

ASJC Scopus subject areas

  • Neuroscience(all)

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Organization of cortical and thalamic input to pyramidal neurons in mouse motor cortex. / Hooks, Bryan M.; Mao, Tianyi; Gutnisky, Diego A.; Yamawaki, Naoki; Svoboda, Karel; Shepherd, Gordon M G.

In: Journal of Neuroscience, Vol. 33, No. 2, 09.01.2013, p. 748-760.

Research output: Contribution to journalArticle

Hooks, Bryan M. ; Mao, Tianyi ; Gutnisky, Diego A. ; Yamawaki, Naoki ; Svoboda, Karel ; Shepherd, Gordon M G. / Organization of cortical and thalamic input to pyramidal neurons in mouse motor cortex. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 2. pp. 748-760.
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