Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation

Nuernisha Alifu, Lulin Yan, Hequn Zhang, Abudureheman Zebibula, Zhenggang Zhu, Wang Xi, Anna Roe, Bin Xu, Wenjing Tian, Jun Qian

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene-co-maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 105 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required.

Original languageEnglish (US)
Pages (from-to)76-85
Number of pages10
JournalDyes and Pigments
Volume143
DOIs
StatePublished - Aug 1 2017
Externally publishedYes

Fingerprint

Biocompatibility
Microscopic examination
Coloring Agents
Photons
Dyes
Maleic anhydride
Nanoparticles
Infrared radiation
Styrene
Fluorescence microscopy
Imaging techniques
Angiography
Light emission
Ultrashort pulses
Contrast Media
Brain
Tissue
poly(styrene-co-maleic anhydride)

Keywords

  • Brain imaging
  • In vivo
  • NIR fluorescence
  • Organic dye nanoparticles
  • Two-photon fluorescence

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

Cite this

Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation. / Alifu, Nuernisha; Yan, Lulin; Zhang, Hequn; Zebibula, Abudureheman; Zhu, Zhenggang; Xi, Wang; Roe, Anna; Xu, Bin; Tian, Wenjing; Qian, Jun.

In: Dyes and Pigments, Vol. 143, 01.08.2017, p. 76-85.

Research output: Contribution to journalArticle

Alifu, Nuernisha ; Yan, Lulin ; Zhang, Hequn ; Zebibula, Abudureheman ; Zhu, Zhenggang ; Xi, Wang ; Roe, Anna ; Xu, Bin ; Tian, Wenjing ; Qian, Jun. / Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation. In: Dyes and Pigments. 2017 ; Vol. 143. pp. 76-85.
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AU - Zebibula, Abudureheman

AU - Zhu, Zhenggang

AU - Xi, Wang

AU - Roe, Anna

AU - Xu, Bin

AU - Tian, Wenjing

AU - Qian, Jun

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AB - Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene-co-maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 105 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required.

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