Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation

Nuernisha Alifu, Lulin Yan, Hequn Zhang, Abudureheman Zebibula, Zhenggang Zhu, Wang Xi, Anna Wang Roe, Bin Xu, Wenjing Tian, Jun Qian

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene-co-maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 105 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required.

Original languageEnglish (US)
Pages (from-to)76-85
Number of pages10
JournalDyes and Pigments
Volume143
DOIs
StatePublished - Aug 1 2017

Keywords

  • Brain imaging
  • In vivo
  • NIR fluorescence
  • Organic dye nanoparticles
  • Two-photon fluorescence

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

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