Abstract
Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene-co-maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 105 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 76-85 |
| Number of pages | 10 |
| Journal | Dyes and Pigments |
| Volume | 143 |
| DOIs | |
| State | Published - Aug 1 2017 |
| Externally published | Yes |
Fingerprint
Keywords
- Brain imaging
- In vivo
- NIR fluorescence
- Organic dye nanoparticles
- Two-photon fluorescence
ASJC Scopus subject areas
- Chemical Engineering(all)
- Process Chemistry and Technology
Cite this
Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation. / Alifu, Nuernisha; Yan, Lulin; Zhang, Hequn; Zebibula, Abudureheman; Zhu, Zhenggang; Xi, Wang; Roe, Anna; Xu, Bin; Tian, Wenjing; Qian, Jun.
In: Dyes and Pigments, Vol. 143, 01.08.2017, p. 76-85.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation
AU - Alifu, Nuernisha
AU - Yan, Lulin
AU - Zhang, Hequn
AU - Zebibula, Abudureheman
AU - Zhu, Zhenggang
AU - Xi, Wang
AU - Roe, Anna
AU - Xu, Bin
AU - Tian, Wenjing
AU - Qian, Jun
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene-co-maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 105 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required.
AB - Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene-co-maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 105 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required.
KW - Brain imaging
KW - In vivo
KW - NIR fluorescence
KW - Organic dye nanoparticles
KW - Two-photon fluorescence
UR - http://www.scopus.com/inward/record.url?scp=85018480968&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018480968&partnerID=8YFLogxK
U2 - 10.1016/j.dyepig.2017.04.017
DO - 10.1016/j.dyepig.2017.04.017
M3 - Article
VL - 143
SP - 76
EP - 85
JO - Dyes and Pigments
JF - Dyes and Pigments
SN - 0143-7208
ER -