Organ culture of human retinal pigment epithelium and choroid: A model for the study of cytologic behavior of RPE in vitro

M. O M Tso, Daniel Albert, L. E. Zimmerman

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Abstract

Organ culture of human choroid and retinal pigment epithelium was maintained in medium RPMI 1640 with Millipore filter as support for various periods up to 6 wk. The tissues were obtained from autopsy and surgical materials. The cultures were studied by light and electron microscopy. The tissues maintained by this culture method appeared to be in a relatively normal active state, without overt degeneration or proliferation for at least 2 wk. The RPE in culture showed capability of phagocytosing particles of thorotrast. Such a culture system serves as a useful model for study of the cytologic behavior of human retinal pigment epithelium (RPE) in vitro, especially during the early stages of disease processes.

Original languageEnglish (US)
Pages (from-to)554-566
Number of pages13
JournalInvestigative ophthalmology
Volume12
Issue number8
Publication statusPublished - Dec 1 1973
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

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