Organ culture of human retinal pigment epithelium and choroid

A model for the study of cytologic behavior of RPE in vitro

M. O M Tso, Daniel Albert, L. E. Zimmerman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Organ culture of human choroid and retinal pigment epithelium was maintained in medium RPMI 1640 with Millipore filter as support for various periods up to 6 wk. The tissues were obtained from autopsy and surgical materials. The cultures were studied by light and electron microscopy. The tissues maintained by this culture method appeared to be in a relatively normal active state, without overt degeneration or proliferation for at least 2 wk. The RPE in culture showed capability of phagocytosing particles of thorotrast. Such a culture system serves as a useful model for study of the cytologic behavior of human retinal pigment epithelium (RPE) in vitro, especially during the early stages of disease processes.

Original languageEnglish (US)
Pages (from-to)554-566
Number of pages13
JournalInvestigative ophthalmology
Volume12
Issue number8
StatePublished - Dec 1 1973
Externally publishedYes

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Choroid
Retinal Pigment Epithelium
Organ Culture Techniques
Thorium Dioxide
Micropore Filters
Phagocytosis
Autopsy
Electron Microscopy
Light
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Organ culture of human retinal pigment epithelium and choroid : A model for the study of cytologic behavior of RPE in vitro. / Tso, M. O M; Albert, Daniel; Zimmerman, L. E.

In: Investigative ophthalmology, Vol. 12, No. 8, 01.12.1973, p. 554-566.

Research output: Contribution to journalArticle

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