Organ culture of human choroid and retinal pigment epithelium was maintained in medium RPMI 1640 with Millipore filter as support for various periods up to 6 wk. The tissues were obtained from autopsy and surgical materials. The cultures were studied by light and electron microscopy. The tissues maintained by this culture method appeared to be in a relatively normal active state, without overt degeneration or proliferation for at least 2 wk. The RPE in culture showed capability of phagocytosing particles of thorotrast. Such a culture system serves as a useful model for study of the cytologic behavior of human retinal pigment epithelium (RPE) in vitro, especially during the early stages of disease processes.
|Original language||English (US)|
|Number of pages||13|
|Publication status||Published - Dec 1 1973|
ASJC Scopus subject areas