Optotaxis: Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient

Felix Hövelmann, Katarzyna M. Kedziora, André Nadler, Rainer Müller, Kees Jalink, Carsten Schultz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Summary Lysophosphatidic acid (LPA) is a serum-borne lipid mediator that binds to a variety of different G protein-coupled receptors to trigger an exceptionally wide range of biological effects, including cell survival and differentiation, cancer cell migration, and embryonic development. Here we synthesized caged LPA (cgLPA), a "photolysable" coumarin-masked derivative of LPA. We demonstrate that illumination of cgLPA with 405 nm light liberates bioactive LPA on a subsecond scale to evoke Ca2+ signaling, Rho activation, and cytoskeletal contraction. In addition, we developed an "optotaxis" assay to attract melanoma cells through a stable chemotactic gradient by repeated liberation of LPA through local photolysis of extracellular cgLPA. We expect that this method of light-controlled chemotaxis will be generally applicable to a large variety of small molecules that drive cellular migration or other responses.

Original languageEnglish (US)
Pages (from-to)629-634
Number of pages6
JournalCell Chemical Biology
Volume23
Issue number5
DOIs
StatePublished - May 19 2016
Externally publishedYes

Fingerprint

Cells
Light
Coumarins
Photolysis
Chemotaxis
G-Protein-Coupled Receptors
Lighting
Embryonic Development
Cell Movement
Cell Differentiation
Melanoma
Assays
Cell Survival
Chemical activation
lysophosphatidic acid
Derivatives
Lipids
Molecules
Serum
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Drug Discovery
  • Pharmacology

Cite this

Optotaxis : Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient. / Hövelmann, Felix; Kedziora, Katarzyna M.; Nadler, André; Müller, Rainer; Jalink, Kees; Schultz, Carsten.

In: Cell Chemical Biology, Vol. 23, No. 5, 19.05.2016, p. 629-634.

Research output: Contribution to journalArticle

Hövelmann, Felix ; Kedziora, Katarzyna M. ; Nadler, André ; Müller, Rainer ; Jalink, Kees ; Schultz, Carsten. / Optotaxis : Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient. In: Cell Chemical Biology. 2016 ; Vol. 23, No. 5. pp. 629-634.
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