TY - JOUR
T1 - Optotaxis
T2 - Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient
AU - Hövelmann, Felix
AU - Kedziora, Katarzyna M.
AU - Nadler, André
AU - Müller, Rainer
AU - Jalink, Kees
AU - Schultz, Carsten
N1 - Funding Information:
This work was partly supported by the DFG ( TRR83 and SFB1129 ) and KWF grant NKI2010-4626 . We are grateful for expert support by EMBL's Advanced Light Microscopy Facility.
Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/5/19
Y1 - 2016/5/19
N2 - Summary Lysophosphatidic acid (LPA) is a serum-borne lipid mediator that binds to a variety of different G protein-coupled receptors to trigger an exceptionally wide range of biological effects, including cell survival and differentiation, cancer cell migration, and embryonic development. Here we synthesized caged LPA (cgLPA), a "photolysable" coumarin-masked derivative of LPA. We demonstrate that illumination of cgLPA with 405 nm light liberates bioactive LPA on a subsecond scale to evoke Ca2+ signaling, Rho activation, and cytoskeletal contraction. In addition, we developed an "optotaxis" assay to attract melanoma cells through a stable chemotactic gradient by repeated liberation of LPA through local photolysis of extracellular cgLPA. We expect that this method of light-controlled chemotaxis will be generally applicable to a large variety of small molecules that drive cellular migration or other responses.
AB - Summary Lysophosphatidic acid (LPA) is a serum-borne lipid mediator that binds to a variety of different G protein-coupled receptors to trigger an exceptionally wide range of biological effects, including cell survival and differentiation, cancer cell migration, and embryonic development. Here we synthesized caged LPA (cgLPA), a "photolysable" coumarin-masked derivative of LPA. We demonstrate that illumination of cgLPA with 405 nm light liberates bioactive LPA on a subsecond scale to evoke Ca2+ signaling, Rho activation, and cytoskeletal contraction. In addition, we developed an "optotaxis" assay to attract melanoma cells through a stable chemotactic gradient by repeated liberation of LPA through local photolysis of extracellular cgLPA. We expect that this method of light-controlled chemotaxis will be generally applicable to a large variety of small molecules that drive cellular migration or other responses.
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U2 - 10.1016/j.chembiol.2015.11.019
DO - 10.1016/j.chembiol.2015.11.019
M3 - Article
C2 - 27161483
AN - SCOPUS:84974824108
SN - 2451-9448
VL - 23
SP - 629
EP - 634
JO - Cell Chemical Biology
JF - Cell Chemical Biology
IS - 5
ER -