Optomotor-blind expression in glial cells is required for correct axonal projection across the Drosophila inner optic chiasm

Kerstin Hofmeyer, Doris Kretzschmar, Gert O. Pflugfelder

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the Drosophila adult visual system, photoreceptor axons and their connecting interneurons are tied into a retinotopic pattern throughout the consecutive neuropil regions: lamina, medulla and lobula complex. Lamina and medulla are joined by the first or outer optic chiasm (OOC). Medulla, lobula and lobula plate are connected by the second or inner optic chiasm (IOC). In the regulatory mutant In(1)ombH31 of the T-box gene optomotor-blind (omb), fibers were found to cross aberrantly through the IOC into the neuropil of the lobula complex. Here, we show that In(1)ombH31 causes selective loss of OMB expression from glial cells within the IOC previously identified as IOC giant glia (ICg-glia). In the absence of OMB, ICg-glia retain their glial cell identity and survive until the adult stage but tend to be displaced into the lobula complex neuropil leading to a misprojection of axons through the IOC. In addition, adult mutant glia show an aberrant increase in length and frequency of glial cell processes. We narrowed down the onset of the IOC defect to the interval between 48 h and 72 h of pupal development. Within the 40 kb of regulatory DNA lacking in In(1)ombH31, we identified an enhancer element (ombC) with activity in the ICg-glia. ombC-driven expression of omb in ICg-glia restored proper axonal projection through the IOC in In(1)ombH31 mutant flies, as well as proper glial cell positioning and morphology. These results indicate that expression of the transcription factor OMB in ICg-glial cells is autonomously required for glial cell migration and morphology and non-autonomously influences axonal pathfinding.

Original languageEnglish (US)
Pages (from-to)28-41
Number of pages14
JournalDevelopmental Biology
Volume315
Issue number1
DOIs
StatePublished - Mar 1 2008

Fingerprint

Optic Chiasm
Neuroglia
Drosophila
Neuropil
Axons
Interneurons
Diptera
Cell Movement
Transcription Factors

Keywords

  • Axonal pathfinding
  • Cell migration
  • Drosophila
  • Glia
  • Gliopodia
  • Optic chiasm
  • Optomotor-blind
  • T-box
  • Visual system development

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Optomotor-blind expression in glial cells is required for correct axonal projection across the Drosophila inner optic chiasm. / Hofmeyer, Kerstin; Kretzschmar, Doris; Pflugfelder, Gert O.

In: Developmental Biology, Vol. 315, No. 1, 01.03.2008, p. 28-41.

Research output: Contribution to journalArticle

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