Optogenetic Control of Mouse Outer Hair Cells

Tao Wu, Sripriya Ramamoorthy, Teresa Wilson, Fangyi Chen, Edward Porsov, Hrebesh Subhash, Sarah Foster, Yuan Zhang, Irina Omelchenko, Michael Bateschell, Lingyan Wang, John Brigande, Zhi-Gen Jiang, Tianyi Mao, Alfred Nuttall

Research output: Contribution to journalArticle

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Abstract

Normal hearing in mammals depends on sound amplification by outer hair cells (OHCs) presumably by their somatic motility and force production. However, the role of OHC force production in cochlear amplification and frequency tuning are not yet fully understood. Currently, available OHC manipulation techniques for physiological or clinical studies are limited by their invasive nature, lack of precision, and poor temporal-spatial resolution. To overcome these limitations, we explored an optogenetic approach based on channelrhodopsin 2 (ChR-2), a direct light-activated nonselective cation channel originally discovered in Chlamydomonas reinhardtii. Three approaches were compared: 1) adeno-associated virus-mediated in utero transfer of the ChR-2 gene into the developing murine otocyst, 2) expression of ChR-2(H134R) in an auditory cell line (HEI-OC1), and 3) expression of ChR-2 in the OHCs of a mouse line carrying a ChR-2 conditional allele. Whole cell recording showed that blue light (470 nm) elicited the typical nonselective cation current of ChR-2 with reversal potential around zero in both mouse OHCs and HEI-OC1 cells and generated depolarization in both cell types. In addition, pulsed light stimulation (10 Hz) elicited a 1:1 repetitive depolarization and ChR-2 currents in mouse OHCs and HEI-OC1 cells, respectively. The time constant of depolarization in OHCs, 1.45 ms, is 10 times faster than HEI-OC1 cells, which allowed light stimulation up to rates of 10/s to elicit corresponding membrane potential changes. Our study demonstrates that ChR-2 can successfully be expressed in mouse OHCs and HEI-OC1 cells and that these present a typical light-sensitive current and depolarization. However, the amount of ChR-2 current induced in our in vivo experiments was insufficient to result in measurable cochlear effects.

Original languageEnglish (US)
Pages (from-to)493-502
Number of pages10
JournalBiophysical Journal
Volume110
Issue number2
DOIs
StatePublished - Jan 19 2016

Fingerprint

Outer Auditory Hair Cells
Optogenetics
Light
Cochlea
Cations
Chlamydomonas reinhardtii
Dependovirus
Patch-Clamp Techniques
Membrane Potentials
Hearing
Mammals

ASJC Scopus subject areas

  • Biophysics

Cite this

Wu, T., Ramamoorthy, S., Wilson, T., Chen, F., Porsov, E., Subhash, H., ... Nuttall, A. (2016). Optogenetic Control of Mouse Outer Hair Cells. Biophysical Journal, 110(2), 493-502. https://doi.org/10.1016/j.bpj.2015.11.3521

Optogenetic Control of Mouse Outer Hair Cells. / Wu, Tao; Ramamoorthy, Sripriya; Wilson, Teresa; Chen, Fangyi; Porsov, Edward; Subhash, Hrebesh; Foster, Sarah; Zhang, Yuan; Omelchenko, Irina; Bateschell, Michael; Wang, Lingyan; Brigande, John; Jiang, Zhi-Gen; Mao, Tianyi; Nuttall, Alfred.

In: Biophysical Journal, Vol. 110, No. 2, 19.01.2016, p. 493-502.

Research output: Contribution to journalArticle

Wu, T, Ramamoorthy, S, Wilson, T, Chen, F, Porsov, E, Subhash, H, Foster, S, Zhang, Y, Omelchenko, I, Bateschell, M, Wang, L, Brigande, J, Jiang, Z-G, Mao, T & Nuttall, A 2016, 'Optogenetic Control of Mouse Outer Hair Cells', Biophysical Journal, vol. 110, no. 2, pp. 493-502. https://doi.org/10.1016/j.bpj.2015.11.3521
Wu T, Ramamoorthy S, Wilson T, Chen F, Porsov E, Subhash H et al. Optogenetic Control of Mouse Outer Hair Cells. Biophysical Journal. 2016 Jan 19;110(2):493-502. https://doi.org/10.1016/j.bpj.2015.11.3521
Wu, Tao ; Ramamoorthy, Sripriya ; Wilson, Teresa ; Chen, Fangyi ; Porsov, Edward ; Subhash, Hrebesh ; Foster, Sarah ; Zhang, Yuan ; Omelchenko, Irina ; Bateschell, Michael ; Wang, Lingyan ; Brigande, John ; Jiang, Zhi-Gen ; Mao, Tianyi ; Nuttall, Alfred. / Optogenetic Control of Mouse Outer Hair Cells. In: Biophysical Journal. 2016 ; Vol. 110, No. 2. pp. 493-502.
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