Optimizing Auditory Brainstem Response Acquisition Using Interleaved Frequencies

Brad N. Buran, Sean Elkins, J. Beth Kempton, Edward V. Porsov, John V. Brigande, Stephen V. David

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Auditory brainstem responses (ABRs) require averaging responses to hundreds or thousands of repetitions of a stimulus (e.g., tone pip) to obtain a measurable evoked response at the scalp. Fast repetition rates lead to changes in ABR amplitude and latency due to adaptation. To minimize the effect of adaptation, stimulus rates are sometimes as low as 10 to 13.3 stimuli per second, requiring long acquisition times. The trade-off between reducing acquisition time and minimizing the effect of adaptation on ABRs is an especially important consideration for studies of cochlear synaptopathy, which use the amplitude of short latency responses (wave 1) to assess auditory nerve survival. It has been proposed that adaptation during ABR acquisition can be reduced by interleaving tones at different frequencies, rather than testing each frequency serially. With careful ordering of frequencies and levels in the stimulus train, adaptation in the auditory nerve can be minimized, thereby permitting an increase in the rate at which tone bursts are presented. However, widespread adoption of this stimulus design has been hindered by lack of available software. Here, we develop and validate an interleaved stimulus design to optimize the rate of ABR measurement while minimizing adaptation. We implement this method in an open-source data acquisition software tool that permits either serial or interleaved ABR measurements. The open-source software library, psiexperiment, is compatible with widely used ABR hardware. Consistent with previous studies, careful design of an interleaved stimulus train can reduce ABR acquisition time by more than half, with minimal effect on ABR thresholds and wave 1 latency, while improving measures of wave 1 amplitude.

Original languageEnglish (US)
Pages (from-to)225-242
Number of pages18
JournalJARO - Journal of the Association for Research in Otolaryngology
Issue number3
StatePublished - Jun 1 2020


  • ABR
  • ABR optimization
  • auditory brainstem response
  • tone burst
  • wave amplitude

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Sensory Systems


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