Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems

Heather M. Mottaz-Brewer, Angela D. Norbeck, Joshua N. Adkins, Nathan P. Manes, Charles Ansong, Liang Shi, Yasuko Rikihisa, Takane Kikuchi, Scott W. Wong, Ryan D. Estep, Fred Heffron, Ljiljana Pasa-Tolic, Richard D. Smith

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Mass spectrometry-based proteomics is a powerful analytical tool for investigating pathogens and their interactions within a host. The sensitivity of such analyses provides broad proteome characterization, but the sample-handling procedures must first be optimized to ensure compatibility with the technique and to maximize the dynamic range of detection. The decision-making process for determining optimal growth conditions, preparation methods, sample analysis methods, and data analysis techniques in our laboratory is discussed herein with consideration of the balance in sensitivity, specificity, and biomass losses during analysis of hostpathogen systems.

Original languageEnglish (US)
Pages (from-to)285-295
Number of pages11
JournalJournal of Biomolecular Techniques
Volume19
Issue number5
StatePublished - Dec 1 2008

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Keywords

  • Mass spectrometry
  • Methods
  • Pathogens
  • Proteomics
  • Sample preparation

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Mottaz-Brewer, H. M., Norbeck, A. D., Adkins, J. N., Manes, N. P., Ansong, C., Shi, L., Rikihisa, Y., Kikuchi, T., Wong, S. W., Estep, R. D., Heffron, F., Pasa-Tolic, L., & Smith, R. D. (2008). Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems. Journal of Biomolecular Techniques, 19(5), 285-295.