Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems

Heather M. Mottaz-Brewer; Angela D. Norbeck; Joshua N. Adkins; Nathan P. Manes; Charles Ansong; Liang Shi; Yasuko Rikihisa; Takane Kikuchi; Scott W. Wong; Ryan D. Estep; Fred Heffron; Ljiljana Pasa-Tolic; Richard D. Smith

Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems

Heather M. Mottaz-Brewer, Angela D. Norbeck, Joshua N. Adkins, Nathan P. Manes, Charles Ansong, Liang Shi, Yasuko Rikihisa, Takane Kikuchi, Scott W. Wong, Ryan D. Estep, Fred Heffron, Ljiljana Pasa-Tolic, Richard D. Smith

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Mass spectrometry-based proteomics is a powerful analytical tool for investigating pathogens and their interactions within a host. The sensitivity of such analyses provides broad proteome characterization, but the sample-handling procedures must first be optimized to ensure compatibility with the technique and to maximize the dynamic range of detection. The decision-making process for determining optimal growth conditions, preparation methods, sample analysis methods, and data analysis techniques in our laboratory is discussed herein with consideration of the balance in sensitivity, specificity, and biomass losses during analysis of hostpathogen systems.

Original language	English (US)
Pages (from-to)	285-295
Number of pages	11
Journal	Journal of Biomolecular Techniques
Volume	19
Issue number	5
State	Published - Dec 2008

Keywords

Mass spectrometry
Methods
Pathogens
Proteomics
Sample preparation

ASJC Scopus subject areas

Molecular Biology

Cite this

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title = "Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems",

abstract = "Mass spectrometry-based proteomics is a powerful analytical tool for investigating pathogens and their interactions within a host. The sensitivity of such analyses provides broad proteome characterization, but the sample-handling procedures must first be optimized to ensure compatibility with the technique and to maximize the dynamic range of detection. The decision-making process for determining optimal growth conditions, preparation methods, sample analysis methods, and data analysis techniques in our laboratory is discussed herein with consideration of the balance in sensitivity, specificity, and biomass losses during analysis of hostpathogen systems.",

keywords = "Mass spectrometry, Methods, Pathogens, Proteomics, Sample preparation",

author = "Mottaz-Brewer, {Heather M.} and Norbeck, {Angela D.} and Adkins, {Joshua N.} and Manes, {Nathan P.} and Charles Ansong and Liang Shi and Yasuko Rikihisa and Takane Kikuchi and Wong, {Scott W.} and Estep, {Ryan D.} and Fred Heffron and Ljiljana Pasa-Tolic and Smith, {Richard D.}",

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language = "English (US)",

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pages = "285--295",

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T1 - Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems

AU - Mottaz-Brewer, Heather M.

AU - Norbeck, Angela D.

AU - Adkins, Joshua N.

AU - Manes, Nathan P.

AU - Ansong, Charles

AU - Shi, Liang

AU - Rikihisa, Yasuko

AU - Kikuchi, Takane

AU - Wong, Scott W.

AU - Estep, Ryan D.

AU - Heffron, Fred

AU - Pasa-Tolic, Ljiljana

AU - Smith, Richard D.

PY - 2008/12

Y1 - 2008/12

N2 - Mass spectrometry-based proteomics is a powerful analytical tool for investigating pathogens and their interactions within a host. The sensitivity of such analyses provides broad proteome characterization, but the sample-handling procedures must first be optimized to ensure compatibility with the technique and to maximize the dynamic range of detection. The decision-making process for determining optimal growth conditions, preparation methods, sample analysis methods, and data analysis techniques in our laboratory is discussed herein with consideration of the balance in sensitivity, specificity, and biomass losses during analysis of hostpathogen systems.

AB - Mass spectrometry-based proteomics is a powerful analytical tool for investigating pathogens and their interactions within a host. The sensitivity of such analyses provides broad proteome characterization, but the sample-handling procedures must first be optimized to ensure compatibility with the technique and to maximize the dynamic range of detection. The decision-making process for determining optimal growth conditions, preparation methods, sample analysis methods, and data analysis techniques in our laboratory is discussed herein with consideration of the balance in sensitivity, specificity, and biomass losses during analysis of hostpathogen systems.

KW - Mass spectrometry

KW - Methods

KW - Pathogens

KW - Proteomics

KW - Sample preparation

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ER -

Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems

Abstract

Keywords

ASJC Scopus subject areas

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