Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds

Hugo P. Sondermeijer, Piotr Witkowski, David Woodland, Tetsunori Seki, Frank J. Aangenendt, Arnoud Van Der Laarse, Silviu Itescu, Mark A. Hardy

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Sodium alginate is an effective biomaterial for tissue engineering applications. Non-purified alginate is contaminated with protein, lipopolysaccharide, DNA, and RNA, which could elicit adverse immunological reactions. We developed a purification protocol to generate biocompatible alginate based on (a) activated charcoal treatment, (b) use of hydrophobic membrane filtration (we used hydrophobic polyvinylidene difluoride membranes to remove organic contaminants), (c) dialysis, and finally (d) ethanol precipitation. Using this approach, we could omit pre-treatment with chloroform and significantly reduce the quantities of reagents used. Purification resulted in reduction of residual protein by 70% down to 0.315 mg/g, DNA by 62% down to 1.28 μg/g, and RNA by 61% down to less than 10 μg/g, respectively. Lipopolysaccharide levels were reduced by >90% to less than 125 EU/g. Purified alginate did not induce splenocyte proliferation in vitro. Three-dimensional scaffolds generated from purified alginate did not elicit a significant foreign body reaction, fibrotic overgrowth, or macrophage infiltration 4 weeks after implantation. This study describes a simplified and economical alginate purification method that results in alginate purity, which meets clinically useful criteria.

Original languageEnglish (US)
Pages (from-to)510-520
Number of pages11
JournalJournal of Biomaterials Applications
Volume31
Issue number4
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Keywords

  • Alginate
  • bioengineering
  • purification
  • scaffolds
  • transplantation

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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