Optimal dye concentration and power density for laser assisted vascular anastomosis (LAVA)

Zhen Ren, Anthony P. Furnary, Hua Xie, Kathryn A. Lagerquist, Allen Burke, Scott Prahl, Kenton Gregory

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser tissue welding with albumin solder/indocyanine green (ICG) dye is an effective technique in surgical reconstruction. This study was carried out in vitro to find optimal ICG concentration and power density (PD) in laser assisted vascular anastomosis (LAVA). Fresh porcine carotid arteries incised into vascular strips (n = 120) were welded by diode laser in end-to-end with 50% albumin solder of 0.01, 0.1, and 1.0 mM ICG and at power density of 27.7, 56.7, and 76.9 W/cm2. Direct temperature was measured by inserting thermocouples outside and inside vessel. Tensile strength was tested immediately and histological study was performed. Temperature (both outside and inside vessel) significantly gradually decreased (p <0.01) with the increasing of ICG concentration at PD 56.7 W/cm2. Tensile strength significantly gradually decreased (p <0.01) with increasing of ICG concentration at PD 56.7 W/cm2. Histological study showed minimal thermal injury limited to adventitia of vessels and no appreciable difference in all groups. We find that ICG concentration within solder is most important factor affecting both tissue temperature and tensile strength during laser vessel welding. The optimal balance between stronger strength and minimal thermal injury of vessel may be achieved primarily by using PD 56.7 W/cm 2 at 0.01 mM ICG within solder during LAVA.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsL.S. Bass, N Kollias, R.S. Malek, A Katzir, U.K. Shah, B.J.F. Wong, E.A. Trowers, T.A. Woodward, W.T.W. de Riese, D.S. Robinson, H-D Reidenbach, K.D. Paulsen, K.W. Gregory
Pages186-193
Number of pages8
Volume4949
DOIs
StatePublished - 2003
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII - San Jose,CA, United States
Duration: Jan 25 2003Jan 26 2003

Other

OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII
CountryUnited States
CitySan Jose,CA
Period1/25/031/26/03

Fingerprint

Soldering alloys
vessels
radiant flux density
solders
Dyes
dyes
Tensile strength
Lasers
tensile strength
lasers
Welding
albumins
welding
Tissue
Thermocouples
Temperature
Semiconductor lasers
thermocouples
arteries
temperature

Keywords

  • Albumin solder
  • Indocyanine green
  • Laser welding
  • Power density
  • Vascular anastomosis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Ren, Z., Furnary, A. P., Xie, H., Lagerquist, K. A., Burke, A., Prahl, S., & Gregory, K. (2003). Optimal dye concentration and power density for laser assisted vascular anastomosis (LAVA). In L. S. Bass, N. Kollias, R. S. Malek, A. Katzir, U. K. Shah, B. J. F. Wong, E. A. Trowers, T. A. Woodward, W. T. W. de Riese, D. S. Robinson, H-D. Reidenbach, K. D. Paulsen, ... K. W. Gregory (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4949, pp. 186-193) https://doi.org/10.1117/12.479374

Optimal dye concentration and power density for laser assisted vascular anastomosis (LAVA). / Ren, Zhen; Furnary, Anthony P.; Xie, Hua; Lagerquist, Kathryn A.; Burke, Allen; Prahl, Scott; Gregory, Kenton.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / L.S. Bass; N Kollias; R.S. Malek; A Katzir; U.K. Shah; B.J.F. Wong; E.A. Trowers; T.A. Woodward; W.T.W. de Riese; D.S. Robinson; H-D Reidenbach; K.D. Paulsen; K.W. Gregory. Vol. 4949 2003. p. 186-193.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ren, Z, Furnary, AP, Xie, H, Lagerquist, KA, Burke, A, Prahl, S & Gregory, K 2003, Optimal dye concentration and power density for laser assisted vascular anastomosis (LAVA). in LS Bass, N Kollias, RS Malek, A Katzir, UK Shah, BJF Wong, EA Trowers, TA Woodward, WTW de Riese, DS Robinson, H-D Reidenbach, KD Paulsen & KW Gregory (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4949, pp. 186-193, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, San Jose,CA, United States, 1/25/03. https://doi.org/10.1117/12.479374
Ren Z, Furnary AP, Xie H, Lagerquist KA, Burke A, Prahl S et al. Optimal dye concentration and power density for laser assisted vascular anastomosis (LAVA). In Bass LS, Kollias N, Malek RS, Katzir A, Shah UK, Wong BJF, Trowers EA, Woodward TA, de Riese WTW, Robinson DS, Reidenbach H-D, Paulsen KD, Gregory KW, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4949. 2003. p. 186-193 https://doi.org/10.1117/12.479374
Ren, Zhen ; Furnary, Anthony P. ; Xie, Hua ; Lagerquist, Kathryn A. ; Burke, Allen ; Prahl, Scott ; Gregory, Kenton. / Optimal dye concentration and power density for laser assisted vascular anastomosis (LAVA). Proceedings of SPIE - The International Society for Optical Engineering. editor / L.S. Bass ; N Kollias ; R.S. Malek ; A Katzir ; U.K. Shah ; B.J.F. Wong ; E.A. Trowers ; T.A. Woodward ; W.T.W. de Riese ; D.S. Robinson ; H-D Reidenbach ; K.D. Paulsen ; K.W. Gregory. Vol. 4949 2003. pp. 186-193
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abstract = "Laser tissue welding with albumin solder/indocyanine green (ICG) dye is an effective technique in surgical reconstruction. This study was carried out in vitro to find optimal ICG concentration and power density (PD) in laser assisted vascular anastomosis (LAVA). Fresh porcine carotid arteries incised into vascular strips (n = 120) were welded by diode laser in end-to-end with 50{\%} albumin solder of 0.01, 0.1, and 1.0 mM ICG and at power density of 27.7, 56.7, and 76.9 W/cm2. Direct temperature was measured by inserting thermocouples outside and inside vessel. Tensile strength was tested immediately and histological study was performed. Temperature (both outside and inside vessel) significantly gradually decreased (p <0.01) with the increasing of ICG concentration at PD 56.7 W/cm2. Tensile strength significantly gradually decreased (p <0.01) with increasing of ICG concentration at PD 56.7 W/cm2. Histological study showed minimal thermal injury limited to adventitia of vessels and no appreciable difference in all groups. We find that ICG concentration within solder is most important factor affecting both tissue temperature and tensile strength during laser vessel welding. The optimal balance between stronger strength and minimal thermal injury of vessel may be achieved primarily by using PD 56.7 W/cm 2 at 0.01 mM ICG within solder during LAVA.",
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