Optimal conditions for detection of reverse transcriptase activity in human placentas

Joann C. Leong; Jay A. Nelson; Jay A. Levy

doi:10.1016/0167-4781(84)90052-6

Optimal conditions for detection of reverse transcriptase activity in human placentas

Joann C. Leong, Jay A. Nelson, Jay A. Levy

Vaccine and Gene Therapy Institute

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Optimal conditions for detecting reverse transcriptase activity in human placental extracts are described. They vary with the state of the placenta at birth and are influenced by relative amounts of detergent, monovalent cation, and protein in the reaction mixture. Demonstrating activity of the placental enzyme requires detergent, but the enzyme is sensitive to high detergent concentrations. This sensitivity can be be altered by lowering the monovalent cation concentration from 0.154 to 0.034 M and by adding protein to the reaction mixture. The detection of reverse transcriptase in Rauscher murine leukemia virus and baboon endogenous type C virus, but not in the Mason-Pfizer monkey type D virus, shows similar requirements.

Original language	English (US)
Pages (from-to)	441-445
Number of pages	5
Journal	BBA - Gene Structure and Expression
Volume	782
Issue number	4
DOIs	https://doi.org/10.1016/0167-4781(84)90052-6
State	Published - Sep 10 1984

Keywords

(Human placenta)
Reverse transcriptase

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(84)90052-6

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abstract = "Optimal conditions for detecting reverse transcriptase activity in human placental extracts are described. They vary with the state of the placenta at birth and are influenced by relative amounts of detergent, monovalent cation, and protein in the reaction mixture. Demonstrating activity of the placental enzyme requires detergent, but the enzyme is sensitive to high detergent concentrations. This sensitivity can be be altered by lowering the monovalent cation concentration from 0.154 to 0.034 M and by adding protein to the reaction mixture. The detection of reverse transcriptase in Rauscher murine leukemia virus and baboon endogenous type C virus, but not in the Mason-Pfizer monkey type D virus, shows similar requirements.",

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T1 - Optimal conditions for detection of reverse transcriptase activity in human placentas

AU - Leong, Joann C.

AU - Nelson, Jay A.

AU - Levy, Jay A.

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N2 - Optimal conditions for detecting reverse transcriptase activity in human placental extracts are described. They vary with the state of the placenta at birth and are influenced by relative amounts of detergent, monovalent cation, and protein in the reaction mixture. Demonstrating activity of the placental enzyme requires detergent, but the enzyme is sensitive to high detergent concentrations. This sensitivity can be be altered by lowering the monovalent cation concentration from 0.154 to 0.034 M and by adding protein to the reaction mixture. The detection of reverse transcriptase in Rauscher murine leukemia virus and baboon endogenous type C virus, but not in the Mason-Pfizer monkey type D virus, shows similar requirements.

AB - Optimal conditions for detecting reverse transcriptase activity in human placental extracts are described. They vary with the state of the placenta at birth and are influenced by relative amounts of detergent, monovalent cation, and protein in the reaction mixture. Demonstrating activity of the placental enzyme requires detergent, but the enzyme is sensitive to high detergent concentrations. This sensitivity can be be altered by lowering the monovalent cation concentration from 0.154 to 0.034 M and by adding protein to the reaction mixture. The detection of reverse transcriptase in Rauscher murine leukemia virus and baboon endogenous type C virus, but not in the Mason-Pfizer monkey type D virus, shows similar requirements.

KW - (Human placenta)

KW - Reverse transcriptase

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Optimal conditions for detection of reverse transcriptase activity in human placentas

Abstract

Keywords

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