Optimal conditions for detection of reverse transcriptase activity in human placentas

Joann C. Leong, Jay Nelson, Jay A. Levy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Optimal conditions for detecting reverse transcriptase activity in human placental extracts are described. They vary with the state of the placenta at birth and are influenced by relative amounts of detergent, monovalent cation, and protein in the reaction mixture. Demonstrating activity of the placental enzyme requires detergent, but the enzyme is sensitive to high detergent concentrations. This sensitivity can be be altered by lowering the monovalent cation concentration from 0.154 to 0.034 M and by adding protein to the reaction mixture. The detection of reverse transcriptase in Rauscher murine leukemia virus and baboon endogenous type C virus, but not in the Mason-Pfizer monkey type D virus, shows similar requirements.

Original languageEnglish (US)
Pages (from-to)441-445
Number of pages5
JournalBBA - Gene Structure and Expression
Volume782
Issue number4
DOIs
StatePublished - Sep 10 1984
Externally publishedYes

Fingerprint

RNA-Directed DNA Polymerase
Viruses
Human Activities
Detergents
Placenta
Monovalent Cations
Mason-Pfizer monkey virus
Placental Extracts
Rauscher Virus
Murine Leukemia Viruses
Papio
Enzymes
Proteins
Parturition

Keywords

  • (Human placenta)
  • Reverse transcriptase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Optimal conditions for detection of reverse transcriptase activity in human placentas. / Leong, Joann C.; Nelson, Jay; Levy, Jay A.

In: BBA - Gene Structure and Expression, Vol. 782, No. 4, 10.09.1984, p. 441-445.

Research output: Contribution to journalArticle

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