Optically characterizing vascular tissue constructs made with soluble vs. homogenized collagen

David Levitz, Monica T. Hinds, Noi T. Tran, Stephen R. Hanson, Steven L. Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability of optical imaging techniques such as optical coherence tomography (OCT) to non-destructively characterize tissue-engineered constructs has generated enormous interest recently. We are testing the hypothesis that OCT data can be used to characterize the cellularity of collagen-based vascular constructs made from 2 types of collagen scaffold matrix: soluble collagen and homogenized collagen. Smooth muscle cells were seeded in these 2 scaffold matrices at a seeding density of 1×106 cells/ml. The disk-shaped constructs were allowed to remodel and compact in the incubator for 96 hours. OCT imaging of the constructs occurred at 24 hour intervals. From the OCT data, the attenuation and reflectivity were evaluated by fitting the data to a theoretical model that relates the tissue optical properties (scattering coefficient and anisotropy factor) and imaging conditions to the OCT signal. The fitted optical properties were compared to the construct volume. Representative H&E histological sections of the constructs were used to assess cell proliferation. Our data showed that the optical properties of the solubilized constructs changed over time while those of the homogenized constructs did not, in agreement with the histology and compaction observations.

Original languageEnglish (US)
Title of host publicationOptics in Tissue Engineering and Regenerative Medicine II
DOIs
StatePublished - Jun 26 2008
EventOptics in Tissue Engineering and Regenerative Medicine II - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6858
ISSN (Print)1605-7422

Other

OtherOptics in Tissue Engineering and Regenerative Medicine II
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

Keywords

  • Anisotropy
  • Cells
  • Collagen
  • Optical coherence tomography
  • Optical properties
  • Scattering
  • Tissue engineering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Levitz, D., Hinds, M. T., Tran, N. T., Hanson, S. R., & Jacques, S. L. (2008). Optically characterizing vascular tissue constructs made with soluble vs. homogenized collagen. In Optics in Tissue Engineering and Regenerative Medicine II [68580E] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6858). https://doi.org/10.1117/12.763713