Optical tissue clearing and immunolabeling in kidney research

Xiao Tong Su, Turgay Saritas, David Ellison

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Optical clearing techniques have recently received wide attention for their potential to reveal microcellular structures deep within tissue. Traditional two-dimensional methods are limited for kidney research since renal tubules, glomeruli, vasculature, and other components exist within three dimensions. Equilibrating the refractive index through thick tissue allows subsequent volumetric imaging. Although optical tissue clearing opens up new doors for kidney research, there remain difficulties to find the most suitable methods and to master most multi-step tissue clearing protocols. Here we present a protocol combining immunolabeling and volumetric imaging, which is simple to implement, does not require specialized equipment and provides a tool to study microanatomy in three dimensions.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
EditorsThomas Weimbs
PublisherAcademic Press Inc.
Pages31-41
Number of pages11
ISBN (Print)9780128203354
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Cell Biology
Volume154
ISSN (Print)0091-679X

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Keywords

  • Immunolabeling
  • Kidney
  • Optical tissue clearing

ASJC Scopus subject areas

  • Cell Biology

Cite this

Su, X. T., Saritas, T., & Ellison, D. (2019). Optical tissue clearing and immunolabeling in kidney research. In T. Weimbs (Ed.), Methods in Cell Biology (pp. 31-41). (Methods in Cell Biology; Vol. 154). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.07.001