Abstract
Separation of the two optical scattering properties, the scattering coefficient (μ s) and the anisotropy of scattering (g), has been experimentally difficult in tissues. A new method for measuring these properties in tissues uses reflectance-mode confocal scanning laser microscopy (rCSLM). Experimentally, the focus at depth z is scanned down into the tissue. The measured data is the exponential decay of the confocal reflectance signal as a function of the depth of the focal volume, R(z)=ρexp(-μz), summarized as a local reflectivity (ρ) and an exponential decay constant (μ). The ρ and μ map uniquely into the μ s and g of the tissue. The method was applied to three mouse skin tissues: one wild-type (wt/wt), one heterozygous mutant (oim/wt), and one homozygous mutant (oim/oim), where oim indicates the mutation for osteogenesis imperfecta, a bone disease that affects type I collagen structure. The mutation affects the collagen fibrils of the skin and the assembly of collagen fiber bundles. The anisotropy of scattering (g) at 488 nm wavelength decreased from 0.81 to 0.46 with the added mutant allele. There was a slight increase in the scattering coefficient (μ s) with the mutation from 74to94cm -1. The decrease in g (toward more isotropic scattering) is likely due to the failure of the mutant fibrils to assemble into the larger collagen fiber bundles that yield forward scattering.
| Original language | English (US) |
|---|---|
| Article number | 041309 |
| Journal | Journal of Biomedical Optics |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2008 |
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Keywords
- anisotropy of scattering
- optical coherence tomography (OCT)
- osteogenesis imperfecta
- reflectance-mode confocal scanning laser microscopy (rCSLM)
- scattering coefficient
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Optical properties of mutant versus wild-type mouse skin measured by reflectance-mode confocal scanning laser microscopy (rCSLM). / Samatham, Ravikant; Jacques, Steven; Campagnola, Paul.
In: Journal of Biomedical Optics, Vol. 13, No. 4, 041309, 2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optical properties of mutant versus wild-type mouse skin measured by reflectance-mode confocal scanning laser microscopy (rCSLM)
AU - Samatham, Ravikant
AU - Jacques, Steven
AU - Campagnola, Paul
PY - 2008
Y1 - 2008
N2 - Separation of the two optical scattering properties, the scattering coefficient (μ s) and the anisotropy of scattering (g), has been experimentally difficult in tissues. A new method for measuring these properties in tissues uses reflectance-mode confocal scanning laser microscopy (rCSLM). Experimentally, the focus at depth z is scanned down into the tissue. The measured data is the exponential decay of the confocal reflectance signal as a function of the depth of the focal volume, R(z)=ρexp(-μz), summarized as a local reflectivity (ρ) and an exponential decay constant (μ). The ρ and μ map uniquely into the μ s and g of the tissue. The method was applied to three mouse skin tissues: one wild-type (wt/wt), one heterozygous mutant (oim/wt), and one homozygous mutant (oim/oim), where oim indicates the mutation for osteogenesis imperfecta, a bone disease that affects type I collagen structure. The mutation affects the collagen fibrils of the skin and the assembly of collagen fiber bundles. The anisotropy of scattering (g) at 488 nm wavelength decreased from 0.81 to 0.46 with the added mutant allele. There was a slight increase in the scattering coefficient (μ s) with the mutation from 74to94cm -1. The decrease in g (toward more isotropic scattering) is likely due to the failure of the mutant fibrils to assemble into the larger collagen fiber bundles that yield forward scattering.
AB - Separation of the two optical scattering properties, the scattering coefficient (μ s) and the anisotropy of scattering (g), has been experimentally difficult in tissues. A new method for measuring these properties in tissues uses reflectance-mode confocal scanning laser microscopy (rCSLM). Experimentally, the focus at depth z is scanned down into the tissue. The measured data is the exponential decay of the confocal reflectance signal as a function of the depth of the focal volume, R(z)=ρexp(-μz), summarized as a local reflectivity (ρ) and an exponential decay constant (μ). The ρ and μ map uniquely into the μ s and g of the tissue. The method was applied to three mouse skin tissues: one wild-type (wt/wt), one heterozygous mutant (oim/wt), and one homozygous mutant (oim/oim), where oim indicates the mutation for osteogenesis imperfecta, a bone disease that affects type I collagen structure. The mutation affects the collagen fibrils of the skin and the assembly of collagen fiber bundles. The anisotropy of scattering (g) at 488 nm wavelength decreased from 0.81 to 0.46 with the added mutant allele. There was a slight increase in the scattering coefficient (μ s) with the mutation from 74to94cm -1. The decrease in g (toward more isotropic scattering) is likely due to the failure of the mutant fibrils to assemble into the larger collagen fiber bundles that yield forward scattering.
KW - anisotropy of scattering
KW - optical coherence tomography (OCT)
KW - osteogenesis imperfecta
KW - reflectance-mode confocal scanning laser microscopy (rCSLM)
KW - scattering coefficient
UR - http://www.scopus.com/inward/record.url?scp=58149382687&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149382687&partnerID=8YFLogxK
U2 - 10.1117/1.2953195
DO - 10.1117/1.2953195
M3 - Article
VL - 13
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 4
M1 - 041309
ER -