TY - JOUR
T1 - Optical monitoring of synaptic vesicle trafficking in ribbon synapses
AU - Guatimosim, Cristina
AU - Von Gersdorff, Henrique
N1 - Funding Information:
This work was supported by a CNPq post-doc fellowship to Cristina Guatimosim and NIH and Pew grants to Henrique von Gersdoff. Cristina Guatimosim wants to thank Dr. Ana Flavia X. Bicalho for the FM1-43 staining scheme.
PY - 2002
Y1 - 2002
N2 - Synaptic transmission constitutes the major basis of communication among nerve cells. Upon nerve terminal depolarisation, calcium influx triggers the exocytosis of synaptic vesicles at active zones. Vesicles are then retrieved by endocytosis, recycled and refilled with neurotransmitter. Fluorescent styryl dyes have proven very useful as tools for studying several aspects of the synaptic vesicle cycle. Here, we review recent imaging studies using styryl FM dyes and bipolar cells of goldfish retina, which have a giant synaptic terminal containing ribbon-type active zones. Optical techniques applied to this unique synaptic terminal have provided novel insights into the trafficking of synaptic vesicles during and following strong stimulation.
AB - Synaptic transmission constitutes the major basis of communication among nerve cells. Upon nerve terminal depolarisation, calcium influx triggers the exocytosis of synaptic vesicles at active zones. Vesicles are then retrieved by endocytosis, recycled and refilled with neurotransmitter. Fluorescent styryl dyes have proven very useful as tools for studying several aspects of the synaptic vesicle cycle. Here, we review recent imaging studies using styryl FM dyes and bipolar cells of goldfish retina, which have a giant synaptic terminal containing ribbon-type active zones. Optical techniques applied to this unique synaptic terminal have provided novel insights into the trafficking of synaptic vesicles during and following strong stimulation.
KW - Fluorescent styryl dyes
KW - Synaptic transmission
KW - Trafficking of synaptic vesicles
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U2 - 10.1016/S0197-0186(02)00015-3
DO - 10.1016/S0197-0186(02)00015-3
M3 - Article
C2 - 12176071
AN - SCOPUS:0035993228
SN - 0197-0186
VL - 41
SP - 307
EP - 312
JO - Neurochemistry International
JF - Neurochemistry International
IS - 5
ER -