Optical Measurements of Presynaptic Release in Mutant Zebrafish Lacking Postsynaptic Receptors

Weiyan Li, Fumihito Ono, Paul Brehm

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Differentiation of presynaptic nerve terminals is mediated, in part, through contact with the appropriate postsynaptic target cell. In particular, studies using dissociated nerve and muscle derived from Xenopus embryos have indicated that the properties of transmitter release from motor neurons are altered after contact with skeletal muscle. This maturation of presynaptic function has further been linked to retrograde signaling from muscle that involves activation of postsynaptic ACh receptors. Using FM1-43 optical determinants of exocytosis, we now compare calcium-mediated exocytosis at neuromuscular junctions of wild-type zebrafish to mutant fish lacking postsynaptic ACh receptors. In response to either high-potassium depolarization or direct electrical stimulation, we observed no differences in the rate or extent of FM1-43 destaining. These data indicate that the acquisition of stimulus-evoked exocytosis at early developmental stages occurs independent of both postsynaptic receptor and synaptic responses in zebrafish.

Original languageEnglish (US)
Pages (from-to)10467-10474
Number of pages8
JournalJournal of Neuroscience
Volume23
Issue number33
StatePublished - Nov 19 2003
Externally publishedYes

Fingerprint

Exocytosis
Zebrafish
Cholinergic Receptors
Muscles
Neurotransmitter Receptor
Neuromuscular Junction
Presynaptic Terminals
Motor Neurons
Xenopus
Electric Stimulation
Potassium
Fishes
Skeletal Muscle
Embryonic Structures
Calcium
FM1 43

Keywords

  • Acetylcholine receptor
  • Exocytosis
  • Motor neuron
  • Mutant
  • Neuromuscular junction
  • Presynaptic differentiation
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Optical Measurements of Presynaptic Release in Mutant Zebrafish Lacking Postsynaptic Receptors. / Li, Weiyan; Ono, Fumihito; Brehm, Paul.

In: Journal of Neuroscience, Vol. 23, No. 33, 19.11.2003, p. 10467-10474.

Research output: Contribution to journalArticle

@article{83e680067cf541a0bc38947032a1a67d,
title = "Optical Measurements of Presynaptic Release in Mutant Zebrafish Lacking Postsynaptic Receptors",
abstract = "Differentiation of presynaptic nerve terminals is mediated, in part, through contact with the appropriate postsynaptic target cell. In particular, studies using dissociated nerve and muscle derived from Xenopus embryos have indicated that the properties of transmitter release from motor neurons are altered after contact with skeletal muscle. This maturation of presynaptic function has further been linked to retrograde signaling from muscle that involves activation of postsynaptic ACh receptors. Using FM1-43 optical determinants of exocytosis, we now compare calcium-mediated exocytosis at neuromuscular junctions of wild-type zebrafish to mutant fish lacking postsynaptic ACh receptors. In response to either high-potassium depolarization or direct electrical stimulation, we observed no differences in the rate or extent of FM1-43 destaining. These data indicate that the acquisition of stimulus-evoked exocytosis at early developmental stages occurs independent of both postsynaptic receptor and synaptic responses in zebrafish.",
keywords = "Acetylcholine receptor, Exocytosis, Motor neuron, Mutant, Neuromuscular junction, Presynaptic differentiation, Zebrafish",
author = "Weiyan Li and Fumihito Ono and Paul Brehm",
year = "2003",
month = "11",
day = "19",
language = "English (US)",
volume = "23",
pages = "10467--10474",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "33",

}

TY - JOUR

T1 - Optical Measurements of Presynaptic Release in Mutant Zebrafish Lacking Postsynaptic Receptors

AU - Li, Weiyan

AU - Ono, Fumihito

AU - Brehm, Paul

PY - 2003/11/19

Y1 - 2003/11/19

N2 - Differentiation of presynaptic nerve terminals is mediated, in part, through contact with the appropriate postsynaptic target cell. In particular, studies using dissociated nerve and muscle derived from Xenopus embryos have indicated that the properties of transmitter release from motor neurons are altered after contact with skeletal muscle. This maturation of presynaptic function has further been linked to retrograde signaling from muscle that involves activation of postsynaptic ACh receptors. Using FM1-43 optical determinants of exocytosis, we now compare calcium-mediated exocytosis at neuromuscular junctions of wild-type zebrafish to mutant fish lacking postsynaptic ACh receptors. In response to either high-potassium depolarization or direct electrical stimulation, we observed no differences in the rate or extent of FM1-43 destaining. These data indicate that the acquisition of stimulus-evoked exocytosis at early developmental stages occurs independent of both postsynaptic receptor and synaptic responses in zebrafish.

AB - Differentiation of presynaptic nerve terminals is mediated, in part, through contact with the appropriate postsynaptic target cell. In particular, studies using dissociated nerve and muscle derived from Xenopus embryos have indicated that the properties of transmitter release from motor neurons are altered after contact with skeletal muscle. This maturation of presynaptic function has further been linked to retrograde signaling from muscle that involves activation of postsynaptic ACh receptors. Using FM1-43 optical determinants of exocytosis, we now compare calcium-mediated exocytosis at neuromuscular junctions of wild-type zebrafish to mutant fish lacking postsynaptic ACh receptors. In response to either high-potassium depolarization or direct electrical stimulation, we observed no differences in the rate or extent of FM1-43 destaining. These data indicate that the acquisition of stimulus-evoked exocytosis at early developmental stages occurs independent of both postsynaptic receptor and synaptic responses in zebrafish.

KW - Acetylcholine receptor

KW - Exocytosis

KW - Motor neuron

KW - Mutant

KW - Neuromuscular junction

KW - Presynaptic differentiation

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=0344011472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344011472&partnerID=8YFLogxK

M3 - Article

C2 - 14627630

AN - SCOPUS:0344011472

VL - 23

SP - 10467

EP - 10474

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 33

ER -