Optical measurement of torsional spring modulus of a double-stranded DNA using half-coated nanoparticles

Jaehyuck Choi, Kai Zhao, Michael J. Heller, Y. H. Lo

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

A technique of detecting the rolling and spinning of half-coated nanoparticles using interference ring patterns of the fluorescence has been applied to the measurement of torsional spring modulus of a double-stranded DNA. Using the unique ability to measure nanoparticle rotations in multiple degrees of freedom, we were able to determine the spinning of a nanoparticle tethered on the DNA and thereby the twisting of the DNA in real time. The detailed knowledge of the spinning as well as rolling behaviors of half-coated nanoparticles provides information about torsional elastic properties of the DNA under investigation.

Original languageEnglish (US)
Pages (from-to)2538-2541
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume26 IV
StatePublished - Dec 1 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004

Keywords

  • Brownian torque
  • DNA
  • Interference ring pattern
  • Nanoparticle
  • Spinning
  • Torsional elastic modulus
  • Twist

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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