Optical measurement of torsional spring modulus of a double-stranded DNA using half-coated nanoparticles

Jaehyuck Choi; Kai Zhao; Michael J. Heller; Y. H. Lo

Optical measurement of torsional spring modulus of a double-stranded DNA using half-coated nanoparticles

Jaehyuck Choi, Kai Zhao, Michael J. Heller, Y. H. Lo

Research output: Contribution to journal › Conference article › peer-review

Abstract

A technique of detecting the rolling and spinning of half-coated nanoparticles using interference ring patterns of the fluorescence has been applied to the measurement of torsional spring modulus of a double-stranded DNA. Using the unique ability to measure nanoparticle rotations in multiple degrees of freedom, we were able to determine the spinning of a nanoparticle tethered on the DNA and thereby the twisting of the DNA in real time. The detailed knowledge of the spinning as well as rolling behaviors of half-coated nanoparticles provides information about torsional elastic properties of the DNA under investigation.

Original language	English (US)
Pages (from-to)	2538-2541
Number of pages	4
Journal	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	26 IV
State	Published - 2004
Externally published	Yes
Event	Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States Duration: Sep 1 2004 → Sep 5 2004

Keywords

Brownian torque
DNA
Interference ring pattern
Nanoparticle
Spinning
Torsional elastic modulus
Twist

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Cite this

Optical measurement of torsional spring modulus of a double-stranded DNA using half-coated nanoparticles. / Choi, Jaehyuck; Zhao, Kai; Heller, Michael J. et al.
In: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Vol. 26 IV, 2004, p. 2538-2541.

Research output: Contribution to journal › Conference article › peer-review

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abstract = "A technique of detecting the rolling and spinning of half-coated nanoparticles using interference ring patterns of the fluorescence has been applied to the measurement of torsional spring modulus of a double-stranded DNA. Using the unique ability to measure nanoparticle rotations in multiple degrees of freedom, we were able to determine the spinning of a nanoparticle tethered on the DNA and thereby the twisting of the DNA in real time. The detailed knowledge of the spinning as well as rolling behaviors of half-coated nanoparticles provides information about torsional elastic properties of the DNA under investigation.",

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journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

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T1 - Optical measurement of torsional spring modulus of a double-stranded DNA using half-coated nanoparticles

AU - Choi, Jaehyuck

AU - Zhao, Kai

AU - Heller, Michael J.

AU - Lo, Y. H.

PY - 2004

Y1 - 2004

N2 - A technique of detecting the rolling and spinning of half-coated nanoparticles using interference ring patterns of the fluorescence has been applied to the measurement of torsional spring modulus of a double-stranded DNA. Using the unique ability to measure nanoparticle rotations in multiple degrees of freedom, we were able to determine the spinning of a nanoparticle tethered on the DNA and thereby the twisting of the DNA in real time. The detailed knowledge of the spinning as well as rolling behaviors of half-coated nanoparticles provides information about torsional elastic properties of the DNA under investigation.

AB - A technique of detecting the rolling and spinning of half-coated nanoparticles using interference ring patterns of the fluorescence has been applied to the measurement of torsional spring modulus of a double-stranded DNA. Using the unique ability to measure nanoparticle rotations in multiple degrees of freedom, we were able to determine the spinning of a nanoparticle tethered on the DNA and thereby the twisting of the DNA in real time. The detailed knowledge of the spinning as well as rolling behaviors of half-coated nanoparticles provides information about torsional elastic properties of the DNA under investigation.

KW - Brownian torque

KW - DNA

KW - Interference ring pattern

KW - Nanoparticle

KW - Spinning

KW - Torsional elastic modulus

KW - Twist

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M3 - Conference article

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SN - 0589-1019

VL - 26 IV

SP - 2538

EP - 2541

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

JF - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

T2 - Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004

Y2 - 1 September 2004 through 5 September 2004

ER -

Optical measurement of torsional spring modulus of a double-stranded DNA using half-coated nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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